TY - JOUR KW - IgY KW - immunity KW - mouse AU - A Selim AU - E Ibrahim AU - M Elhaig AB - An anti-Giardia lamblia (G. lamblia) antibody in chicken egg yolk (anti-GL IgY) was developed and its in vitro and in vivo effectiveness was investigated. Anti-GL IgY expressed the ability to agglutinate Giardia lamblia trophozoites with significant reduction of their movement. In addition, IgY reduced the attachment capacity of G. lamblia trophozoites to intestinal cells in an in vitro attachment inhibition assay in a dose-dependent manner. The protective efficacy of anti-GL IgY was investigated in experimentally induced infection in mice following oral application of G. lamblia trophozoites. Anti-GL IgY improved the body weight gain of the mice in comparison to the infected group. The output of cysts was reduced significantly. These results indicate that anti-GL IgY has a protective effect against experimentally induced giardiasis in mice. This effect might be due to blocking the binding of G. lamblia to the host cells. We show the first evidences about the effect of a specific antibody developed in chicken egg yolk on the protozoan G. lamblia. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":100028,"title":"Passive protection against Giardia lamblia infection by specific immunoglobulins from chicken egg yolk","topline":"","teaserText":"Passiver Schutz gegen eine Infektion mit Gardia lamblia durch spezifische Immunglobuline aus H\u00fchnereidotter","content":"

Summary<\/span>
An anti-Giardia lamblia (G. lamblia) antibody in chicken egg yolk (anti-GL IgY) was developed and its in vitro and in vivo effectiveness was investigated. Anti-GL IgY expressed the ability to agglutinate Giardia lamblia trophozoites with significant reduction of their movement. In addition, IgY reduced the attachment capacity of G. lamblia trophozoites to intestinal cells in an in vitro attachment inhibition assay in a dose-dependent manner. The protective efficacy of anti-GL IgY was investigated in experimentally induced infection in mice following oral application of G. lamblia trophozoites. Anti-GL IgY improved the body weight gain of the mice in comparison to the infected group. The output of cysts was reduced significantly. These results indicate that anti-GL IgY has a protective effect against experimentally induced giardiasis in mice. This effect might be due to blocking the binding of G. lamblia to the host cells. We show the first evidences about the effect of a specific antibody developed in chicken egg yolk on the protozoan G. lamblia.<\/p>

Keywords<\/span>
IgY, immunity, mouse<\/p>

Zusammenfassung<\/span>
Ein anti-Giardia lamblia-Antik\u00f6rper (anti-GL IgY) wurde aus H\u00fchnereidotter entwickelt und dessen Wirksamkeit in vitro und in vivo untersucht. Anti- GL IgY konnten Giardia (G.) lamblia-Trophozoiten agglutinieren und deren Be- wegungen signifikant reduzieren. Weiterhin zeigte ein In-vitro-Bindungs-Inhibitions-Assay, dass IgY die Bindungsf\u00e4higkeit der G. lamblia-Trophozoiten an intestinale Zellen dosisabh\u00e4ngig mindert. Die Schutzwirkung der anti-GL IgY wurde in einer experimentellen Infektion von M\u00e4usen durch orale Applikation von G. lamblia-Trophozoiten untersucht. Anti-GL IgY erh\u00f6hte die Gewichtszunahme dieser M\u00e4use im Vergleich mit den infizierten M\u00e4usen, die keine Anti- GL IgY erhielten. Die Produktion von Zysten war signifikant reduziert. Die Ergebnisse deuten auf eine Schutzwirkung der anti-GL IgY bei einer experimentellen Giardiose von M\u00e4usen hin, welche durch Inhibierung der Bindung der G. lamblia an die Wirtszellen bedingt sein kann. Diese Studie erbringt den ersten Nachweis \u00fcber die Wirkung eines in Eidotter entwickelten spezifischen Antik\u00f6rpers gegen den Einzeller G. lamblia.<\/p>

Schl\u00fcsselw\u00f6rter<\/span>
IgY, Immunit\u00e4t, Maus<\/p>","categories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"],"fromDate":"Jan 14, 2017 11:00:00 PM","oldUrls":["http:\/\/vetline.de\/passive-protection-against-giardia-lamblia-infection-by-specific-immunoglobulins-from-chicken-egg-yolk\/150\/3130\/100028"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","doiDocumentUri":"http:\/\/www.vetline.de\/passive-protection-against-giardia-lamblia-infection-by-specific-immunoglobulins-from-chicken-egg-yolk\/150\/3130\/100028\/","doiSource":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift 130, Heft 1\/2 (2017), Seiten 78\u201385","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-15126","doiFirstPage":"78","doiLastPage":"85","doiTransmitted":true,"doiAuthor":"Selim A, Ibrahim E, Elhaig M","pdf":{"path":"http:\/\/data\/BMW_2017_01_02_0078.pdf","title":"BMW_2017_01_02_0078.pdf","description":"Passive protection against Giardia lamblia infection by specific immunoglobulins from chicken egg yolk"},"authors":[{"firstName":"A","middleName":"","lastName":"Selim"},{"firstName":"E","middleName":"","lastName":"Ibrahim"},{"firstName":"M","middleName":"","lastName":"Elhaig"}],"contentOptimised":"

Summary<\/strong>
An anti-Giardia lamblia (G. lamblia) antibody in chicken egg yolk (anti-GL IgY) was developed and its in vitro and in vivo effectiveness was investigated. Anti-GL IgY expressed the ability to agglutinate Giardia lamblia trophozoites with significant reduction of their movement. In addition, IgY reduced the attachment capacity of G. lamblia trophozoites to intestinal cells in an in vitro attachment inhibition assay in a dose-dependent manner. The protective efficacy of anti-GL IgY was investigated in experimentally induced infection in mice following oral application of G. lamblia trophozoites. Anti-GL IgY improved the body weight gain of the mice in comparison to the infected group. The output of cysts was reduced significantly. These results indicate that anti-GL IgY has a protective effect against experimentally induced giardiasis in mice. This effect might be due to blocking the binding of G. lamblia to the host cells. We show the first evidences about the effect of a specific antibody developed in chicken egg yolk on the protozoan G. lamblia.<\/p>

Keywords:<\/strong>
IgY, immunity, mouse<\/p>

Zusammenfassung<\/strong>
Ein anti-Giardia lamblia-Antik\u00f6rper (anti-GL IgY) wurde aus H\u00fchnereidotter entwickelt und dessen Wirksamkeit in vitro und in vivo untersucht. Anti- GL IgY konnten Giardia (G.) lamblia-Trophozoiten agglutinieren und deren Be- wegungen signifikant reduzieren. Weiterhin zeigte ein In-vitro-Bindungs-Inhibitions-Assay, dass IgY die Bindungsf\u00e4higkeit der G. lamblia-Trophozoiten an intestinale Zellen dosisabh\u00e4ngig mindert. Die Schutzwirkung der anti-GL IgY wurde in einer experimentellen Infektion von M\u00e4usen durch orale Applikation von G. lamblia-Trophozoiten untersucht. Anti-GL IgY erh\u00f6hte die Gewichtszunahme dieser M\u00e4use im Vergleich mit den infizierten M\u00e4usen, die keine Anti- GL IgY erhielten. Die Produktion von Zysten war signifikant reduziert. Die Ergebnisse deuten auf eine Schutzwirkung der anti-GL IgY bei einer experimentellen Giardiose von M\u00e4usen hin, welche durch Inhibierung der Bindung der G. lamblia an die Wirtszellen bedingt sein kann. Diese Studie erbringt den ersten Nachweis \u00fcber die Wirkung eines in Eidotter entwickelten spezifischen Antik\u00f6rpers gegen den Einzeller G. lamblia.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
IgY, Immunit\u00e4t, Maus<\/p>","primaryLanguage":"englisch","summary":"An anti-Giardia lamblia (G. lamblia) antibody in chicken egg yolk (anti-GL IgY) was developed and its in vitro and in vivo effectiveness was investigated. Anti-GL IgY expressed the ability to agglutinate Giardia lamblia trophozoites with significant reduction of their movement. In addition, IgY reduced the attachment capacity of G. lamblia trophozoites to intestinal cells in an in vitro attachment inhibition assay in a dose-dependent manner. The protective efficacy of anti-GL IgY was investigated in experimentally induced infection in mice following oral application of G. lamblia trophozoites. Anti-GL IgY improved the body weight gain of the mice in comparison to the infected group. The output of cysts was reduced significantly. These results indicate that anti-GL IgY has a protective effect against experimentally induced giardiasis in mice. This effect might be due to blocking the binding of G. lamblia to the host cells. We show the first evidences about the effect of a specific antibody developed in chicken egg yolk on the protozoan G. lamblia.<\/p>

","keywords":["IgY","immunity","mouse"],"zusammenfassung":"Ein anti-Giardia lamblia-Antik\u00f6rper (anti-GL IgY) wurde aus H\u00fchnereidotter entwickelt und dessen Wirksamkeit in vitro und in vivo untersucht. Anti- GL IgY konnten Giardia (G.) lamblia-Trophozoiten agglutinieren und deren Be- wegungen signifikant reduzieren. Weiterhin zeigte ein In-vitro-Bindungs-Inhibitions-Assay, dass IgY die Bindungsf\u00e4higkeit der G. lamblia-Trophozoiten an intestinale Zellen dosisabh\u00e4ngig mindert. Die Schutzwirkung der anti-GL IgY wurde in einer experimentellen Infektion von M\u00e4usen durch orale Applikation von G. lamblia-Trophozoiten untersucht. Anti-GL IgY erh\u00f6hte die Gewichtszunahme dieser M\u00e4use im Vergleich mit den infizierten M\u00e4usen, die keine Anti- GL IgY erhielten. Die Produktion von Zysten war signifikant reduziert. Die Ergebnisse deuten auf eine Schutzwirkung der anti-GL IgY bei einer experimentellen Giardiose von M\u00e4usen hin, welche durch Inhibierung der Bindung der G. lamblia an die Wirtszellen bedingt sein kann. Diese Studie erbringt den ersten Nachweis \u00fcber die Wirkung eines in Eidotter entwickelten spezifischen Antik\u00f6rpers gegen den Einzeller G. lamblia.<\/p>

","schluesselwoerter":["IgY","Immunit\u00e4t","Maus"],"translatedTitle":"Passiver Schutz gegen eine Infektion mit Gardia lamblia durch spezifische Immunglobuline aus H\u00fchnereidotter","abstractE":"An anti-Giardia lamblia (G. lamblia) antibody in chicken egg yolk (anti-GL IgY) was developed and its in vitro and in vivo effectiveness was investigated. Anti-GL IgY expressed the ability to agglutinate Giardia lamblia trophozoites with significant reduction of their movement. In addition, IgY reduced the attachment capacity of G. lamblia trophozoites to intestinal cells in an in vitro attachment inhibition assay in a dose-dependent manner. The protective efficacy of anti-GL IgY was investigated in experimentally induced infection in mice following oral application of G. lamblia trophozoites. Anti-GL IgY improved the body weight gain of the mice in comparison to the infected group. The output of cysts was reduced significantly. These results indicate that anti-GL IgY has a protective effect against experimentally induced giardiasis in mice. This effect might be due to blocking the binding of G. lamblia to the host cells. We show the first evidences about the effect of a specific antibody developed in chicken egg yolk on the protozoan G. lamblia.","date":{"year":2017,"date":"01\/2017","accepted":"2017-01-14"},"volume":"130","openAccess":false,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"78-85","redirects":["passive-protection-against-giardia-lamblia-infection-by-specific-immunoglobulins-from-chicken-egg-yolk\/150\/3130\/100028"],"tierartCategories":[],"artikelartCategories":["Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel"]} CY - Hannover DA - 01/2017 DO - 10.2376/0005-9366-15126 ET - 1/2 LA - English N2 - An anti-Giardia lamblia (G. lamblia) antibody in chicken egg yolk (anti-GL IgY) was developed and its in vitro and in vivo effectiveness was investigated. Anti-GL IgY expressed the ability to agglutinate Giardia lamblia trophozoites with significant reduction of their movement. In addition, IgY reduced the attachment capacity of G. lamblia trophozoites to intestinal cells in an in vitro attachment inhibition assay in a dose-dependent manner. The protective efficacy of anti-GL IgY was investigated in experimentally induced infection in mice following oral application of G. lamblia trophozoites. Anti-GL IgY improved the body weight gain of the mice in comparison to the infected group. The output of cysts was reduced significantly. These results indicate that anti-GL IgY has a protective effect against experimentally induced giardiasis in mice. This effect might be due to blocking the binding of G. lamblia to the host cells. We show the first evidences about the effect of a specific antibody developed in chicken egg yolk on the protozoan G. lamblia. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2017 SP - 78 EP - 85 T1 - Passive protection against Giardia lamblia infection by specific immunoglobulins from chicken egg yolk T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Passive protection against Giardia lamblia infection by specific immunoglobulins from chicken egg yolk TT - Passiver Schutz gegen eine Infektion mit Gardia lamblia durch spezifische Immunglobuline aus Hühnereidotter VL - 130 SN - 0005-9366 ER -