TY - JOUR KW - real-time PCR KW - metabolic state KW - detection methods KW - bacterial death KW - PMA KW - EMA AU - K Stingl AU - C Buhler AU - N Krüger AB - Death, although absolute in its consequence, is not measurable by an absolute parameter in bacteria. Viability assays address different aspects of life, e. g. the capability to form a colony on an agar plate (CFU), metabolic properties or membrane integrity. For food safety, presence of infectious potential is the relevant criterion for risk assessment, currently only partly reflected by the quantification of CFU. It will be necessary for future improved risk assessment, in particular when fastidious bacterial pathogens are implicated, to enhance the informative value of CFU. This might be feasible by quantification of the number of intact and potentially infectious Campylobacter, impermeable to the DNA intercalating dye propidium monoazide (PMA). The latter are quantifiable by the combination of PMA with real-time PCR, although thorough controls have to be developed for standardization and the circumvention of pitfalls. Under consideration of different physiological states of the food-borne pathogen, we provide an overview of current and future suitable detection/quantification targets along the food chain, including putative limitations of detection. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":83479,"title":"Campylobacter detection along the food chain \u2013 towards improved quantitative risk analysis by live\/dead discriminatory culture-independent methods","topline":"Open Access | CARO-Themenheft","teaserText":"Detektion von Campylobacter entlang der Lebensmittelkette \u2013 auf dem Weg zu einer verbesserten quantitativen Risikoanalyse durch lebend\/tot unterscheidende, kultivierungsunabh\u00e4ngige Methoden","content":"

Summary <\/span>
Death, although absolute in its consequence, is not measurable by an absolute parameter in bacteria. Viability assays address different aspects of life, e. g. the capability to form a colony on an agar plate (CFU), metabolic properties or membrane integrity. For food safety, presence of infectious potential is the relevant criterion for risk assessment, currently only partly reflected by the quantification of CFU. It will be necessary for future improved risk assessment, in particular when fastidious bacterial pathogens are implicated, to enhance the informative value of CFU. This might be feasible by quantification of the number of intact and potentially infectious Campylobacter<\/span>, impermeable to the DNA intercalating dye propidium monoazide (PMA). The latter are quantifiable by the combination of PMA with real-time PCR, although thorough controls have to be developed for standardization and the circumvention of pitfalls. Under consideration of different physiological states of the food-borne pathogen, we provide an overview of current and future suitable detection\/quantification targets along the food chain, including putative limitations of detection. <\/p>

Keywords: <\/span>
real-time PCR, metabolic state, detection methods, bacterial death, PMA, EMA<\/p>

Zusammenfassung <\/span>
Der Tod, wenngleich in seiner Konsequenz absolut, ist bei Bakterien nicht absolut messbar. Untersuchungen zur Lebensf\u00e4higkeit zielen auf verschiedene Aspekte
des Lebens, z. B. die F\u00e4higkeit, eine Kolonie auf einer Platte zu bilden (KbE), metabolische Eigenschaften oder die Integrit\u00e4t der Membran. F\u00fcr die Lebensmittelsicherheit
stellt das relevante Kriterium f\u00fcr Risikobewertung das infekti\u00f6se Potenzial dar, welches zurzeit nur teilweise durch die Quantifizierung der KbE repr\u00e4sentiert
wird. Insbesondere bei anspruchsvollen bakteriellen Pathogenen wird es zuk\u00fcnftig f\u00fcr eine verbesserte Risikobewertung notwendig sein, den Informationsgehalt
der KbE zu erweitern. Dies k\u00f6nnte durch die Quantifizierung intakter und potentiell infekti\u00f6ser Campylobacter <\/span>realisiert werden, welche impermeabel f\u00fcr den in
DNA interkalierenden Farbstoff Propidium-Monoazid (PMA) sind. Diese sind durch die Kombination von PMA mit Real-time PCR quantifizierbar, obwohl sorgf\u00e4ltige
Kontrollen zur Standardisierung und f\u00fcr das Umgehen von Artefakten entwickelt werden m\u00fcssen. Unter Ber\u00fccksichtigung der verschiedenen physiologischen
Zust\u00e4nde des Lebensmittel-Pathogens, geben wir eine \u00dcbersicht \u00fcber die derzeitigen und zuk\u00fcnftigen geeigneten Detektions-\/Quantifizierungs-Zielparameter entlang der Lebensmittelkette, einschlie\u00dflich m\u00f6glicher Detektionsgrenzen. <\/p>

Schl\u00fcsselw\u00f6rter: <\/span>
Real-time PCR, metabolischer Zustand, Detektionsmethoden, bakterieller Tod, PMA, EMA<\/p>","categories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"],"fromDate":"Nov 19, 2014 2:28:41 PM","oldUrls":["http:\/\/vetline.de\/campylobacter-detection-along-the-food-chain\/150\/3130\/83479","http:\/\/vetline.de\/campylobacter-detection-along-the-food-chain\/150\/3216\/83479"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl M\u00fcnch Tier\u00e4rztl Wochenschr","doiDocumentUri":"http:\/\/www.vetline.de\/campylobacter-detection-along-the-food-chain\/150\/3216\/83479\/","doiSource":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG 128, 122\u2013129","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-128-122","doiFirstPage":"122","doiLastPage":"129","doiTransmitted":true,"doiAuthor":"Stingl K, Buhler C, Kr\u00fcger N","pdf":{"path":"http:\/\/data\/BMW_2015_0122.pdf","title":"BMW_2015_0122","description":"Campylobacter detection along the food chain \u2013 towards improved quantitative risk analysis by live\/dead discriminatory culture-independent methods"},"authors":[{"firstName":"K","middleName":"","lastName":"Stingl"},{"firstName":"C","middleName":"","lastName":"Buhler"},{"firstName":"N","middleName":"","lastName":"Kr\u00fcger"}],"contentOptimised":"

Summary<\/strong>
Death, although absolute in its consequence, is not measurable by an absolute parameter in bacteria. Viability assays address different aspects of life, e. g. the capability to form a colony on an agar plate (CFU), metabolic properties or membrane integrity. For food safety, presence of infectious potential is the relevant criterion for risk assessment, currently only partly reflected by the quantification of CFU. It will be necessary for future improved risk assessment, in particular when fastidious bacterial pathogens are implicated, to enhance the informative value of CFU. This might be feasible by quantification of the number of intact and potentially infectious Campylobacter<\/strong>, impermeable to the DNA intercalating dye propidium monoazide (PMA). The latter are quantifiable by the combination of PMA with real-time PCR, although thorough controls have to be developed for standardization and the circumvention of pitfalls. Under consideration of different physiological states of the food-borne pathogen, we provide an overview of current and future suitable detection\/quantification targets along the food chain, including putative limitations of detection. <\/p>

Keywords:<\/strong>
real-time PCR, metabolic state, detection methods, bacterial death, PMA, EMA<\/p>

Zusammenfassung<\/strong>
Der Tod, wenngleich in seiner Konsequenz absolut, ist bei Bakterien nicht absolut messbar. Untersuchungen zur Lebensf\u00e4higkeit zielen auf verschiedene Aspekte
des Lebens, z. B. die F\u00e4higkeit, eine Kolonie auf einer Platte zu bilden (KbE), metabolische Eigenschaften oder die Integrit\u00e4t der Membran. F\u00fcr die Lebensmittelsicherheit
stellt das relevante Kriterium f\u00fcr Risikobewertung das infekti\u00f6se Potenzial dar, welches zurzeit nur teilweise durch die Quantifizierung der KbE repr\u00e4sentiert
wird. Insbesondere bei anspruchsvollen bakteriellen Pathogenen wird es zuk\u00fcnftig f\u00fcr eine verbesserte Risikobewertung notwendig sein, den Informationsgehalt
der KbE zu erweitern. Dies k\u00f6nnte durch die Quantifizierung intakter und potentiell infekti\u00f6ser Campylobacter <\/em>realisiert werden, welche impermeabel f\u00fcr den in
DNA interkalierenden Farbstoff Propidium-Monoazid (PMA) sind. Diese sind durch die Kombination von PMA mit Real-time PCR quantifizierbar, obwohl sorgf\u00e4ltige
Kontrollen zur Standardisierung und f\u00fcr das Umgehen von Artefakten entwickelt werden m\u00fcssen. Unter Ber\u00fccksichtigung der verschiedenen physiologischen
Zust\u00e4nde des Lebensmittel-Pathogens, geben wir eine \u00dcbersicht \u00fcber die derzeitigen und zuk\u00fcnftigen geeigneten Detektions-\/Quantifizierungs-Zielparameter entlang der Lebensmittelkette, einschlie\u00dflich m\u00f6glicher Detektionsgrenzen. <\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
Real-time PCR, metabolischer Zustand, Detektionsmethoden, bakterieller Tod, PMA, EMA<\/p>","primaryLanguage":"englisch","summary":"Death, although absolute in its consequence, is not measurable by an absolute parameter in bacteria. Viability assays address different aspects of life, e. g. the capability to form a colony on an agar plate (CFU), metabolic properties or membrane integrity. For food safety, presence of infectious potential is the relevant criterion for risk assessment, currently only partly reflected by the quantification of CFU. It will be necessary for future improved risk assessment, in particular when fastidious bacterial pathogens are implicated, to enhance the informative value of CFU. This might be feasible by quantification of the number of intact and potentially infectious Campylobacter<\/strong>, impermeable to the DNA intercalating dye propidium monoazide (PMA). The latter are quantifiable by the combination of PMA with real-time PCR, although thorough controls have to be developed for standardization and the circumvention of pitfalls. Under consideration of different physiological states of the food-borne pathogen, we provide an overview of current and future suitable detection\/quantification targets along the food chain, including putative limitations of detection. <\/p>

","keywords":["real-time PCR","metabolic state","detection methods","bacterial death","PMA","EMA"],"zusammenfassung":"Der Tod, wenngleich in seiner Konsequenz absolut, ist bei Bakterien nicht absolut messbar. Untersuchungen zur Lebensf\u00e4higkeit zielen auf verschiedene Aspekte
des Lebens, z. B. die F\u00e4higkeit, eine Kolonie auf einer Platte zu bilden (KbE), metabolische Eigenschaften oder die Integrit\u00e4t der Membran. F\u00fcr die Lebensmittelsicherheit
stellt das relevante Kriterium f\u00fcr Risikobewertung das infekti\u00f6se Potenzial dar, welches zurzeit nur teilweise durch die Quantifizierung der KbE repr\u00e4sentiert
wird. Insbesondere bei anspruchsvollen bakteriellen Pathogenen wird es zuk\u00fcnftig f\u00fcr eine verbesserte Risikobewertung notwendig sein, den Informationsgehalt
der KbE zu erweitern. Dies k\u00f6nnte durch die Quantifizierung intakter und potentiell infekti\u00f6ser Campylobacter <\/em>realisiert werden, welche impermeabel f\u00fcr den in
DNA interkalierenden Farbstoff Propidium-Monoazid (PMA) sind. Diese sind durch die Kombination von PMA mit Real-time PCR quantifizierbar, obwohl sorgf\u00e4ltige
Kontrollen zur Standardisierung und f\u00fcr das Umgehen von Artefakten entwickelt werden m\u00fcssen. Unter Ber\u00fccksichtigung der verschiedenen physiologischen
Zust\u00e4nde des Lebensmittel-Pathogens, geben wir eine \u00dcbersicht \u00fcber die derzeitigen und zuk\u00fcnftigen geeigneten Detektions-\/Quantifizierungs-Zielparameter entlang der Lebensmittelkette, einschlie\u00dflich m\u00f6glicher Detektionsgrenzen. <\/p>

","schluesselwoerter":["Real-time PCR","metabolischer Zustand","Detektionsmethoden","bakterieller Tod","PMA","EMA"],"translatedTitle":"Detektion von Campylobacter entlang der Lebensmittelkette \u2013 auf dem Weg zu einer verbesserten quantitativen Risikoanalyse durch lebend\/tot unterscheidende, kultivierungsunabh\u00e4ngige Methoden","abstractE":"Death, although absolute in its consequence, is not measurable by an absolute parameter in bacteria. Viability assays address different aspects of life, e. g. the capability to form a colony on an agar plate (CFU), metabolic properties or membrane integrity. For food safety, presence of infectious potential is the relevant criterion for risk assessment, currently only partly reflected by the quantification of CFU. It will be necessary for future improved risk assessment, in particular when fastidious bacterial pathogens are implicated, to enhance the informative value of CFU. This might be feasible by quantification of the number of intact and potentially infectious Campylobacter, impermeable to the DNA intercalating dye propidium monoazide (PMA). The latter are quantifiable by the combination of PMA with real-time PCR, although thorough controls have to be developed for standardization and the circumvention of pitfalls. Under consideration of different physiological states of the food-borne pathogen, we provide an overview of current and future suitable detection\/quantification targets along the food chain, including putative limitations of detection. ","date":{"year":2014,"date":"11\/2014","accepted":"2014-11-19"},"volume":"128","openAccess":true,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"122-129","redirects":["campylobacter-detection-along-the-food-chain\/150\/3130\/83479","campylobacter-detection-along-the-food-chain\/150\/3216\/83479"],"tierartCategories":[],"artikelartCategories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"]} CY - Hannover DA - 11/2014 DO - 10.2376/0005-9366-128-122 ET - 3/4 LA - English N2 - Death, although absolute in its consequence, is not measurable by an absolute parameter in bacteria. Viability assays address different aspects of life, e. g. the capability to form a colony on an agar plate (CFU), metabolic properties or membrane integrity. For food safety, presence of infectious potential is the relevant criterion for risk assessment, currently only partly reflected by the quantification of CFU. It will be necessary for future improved risk assessment, in particular when fastidious bacterial pathogens are implicated, to enhance the informative value of CFU. This might be feasible by quantification of the number of intact and potentially infectious Campylobacter, impermeable to the DNA intercalating dye propidium monoazide (PMA). The latter are quantifiable by the combination of PMA with real-time PCR, although thorough controls have to be developed for standardization and the circumvention of pitfalls. Under consideration of different physiological states of the food-borne pathogen, we provide an overview of current and future suitable detection/quantification targets along the food chain, including putative limitations of detection. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2014 SP - 122 EP - 129 T1 - Campylobacter detection along the food chain – towards improved quantitative risk analysis by live/dead discriminatory culture-independent methods T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Campylobacter detection along the food chain – towards improved quantitative risk analysis by live/dead discriminatory culture-independent methods TT - Detektion von Campylobacter entlang der Lebensmittelkette – auf dem Weg zu einer verbesserten quantitativen Risikoanalyse durch lebend/tot unterscheidende, kultivierungsunabhängige Methoden VL - 128 SN - 0005-9366 ER -