TY - JOUR KW - Fluoroquinolone KW - resistance KW - route KW - transfer AU - E Burow AU - M Grobbel AU - B-A Tenhagen AU - C Simoneit AU - M Ladwig AU - I Szabo AU - D Wendt AU - S Banneke AU - A Käsbohrer AB - The study objective was to evaluate the effect of oral (OT) and parenteral (PT) administration of enrofloxacin to weaners on untreated contact animals. We assessed a) fluoroquinolone occurrence in the blood serum of untreated contact animals (COT, CPT); b) resistance to (fluoro)quinolones in commensal Escherichia coli (E. coli) in OT, PT, COT and CPT compared to the control (CON), and c) resistance to other antimicrobials in E. coli in OT, PT, COT and CPT compared to the initial situation before the treatment in these groups. Five groups of 14 weaners each were housed in three separate rooms (OT with COT, PT with CPT, CON alone). OT and PT were treated with enrofloxacin for five days. Rectal swabs and blood samples were taken before, during and until 51 days after treatment. Enro- and ciprofloxacin were detected in all treated, all COT and half of the CPT pigs. Neither through selective isolation nor by susceptibility testing of one random non-selectively isolated faecal E. coli per sample, resistance to ciprofloxacin (metabolite of enrofloxacin) and nalidixic acid was detected in both treatment and contact groups during and short after treatment. However, a transient increase of E. coli resistant to antimicrobials other than quinolones followed the treatment in isolates from OT and COT (e.g. ampicillin p lt; 0.05). In conclusion, animals in contact with treated animals are exposed to and can intake antimicrobials. Animals in contact with orally treated animals show occurrence of antibiotic resistant E. coli. Further studies are needed to show whether these preliminary findings can be confirmed under different conditions and with more sensitive detection methods. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":107369,"title":"Antimicrobial susceptibility in faecal Escherichia coli from pigs after enrofloxacin administration in an experimental environment","topline":"","teaserText":"Empfindlichkeit f\u00e4kaler Escherichia coli von Schweinen gegen\u00fcber Antibiotika nach Enrofloxacinverabreichung in einer experimentellen Umgebung","content":"

Summary<\/span>
The study objective was to evaluate the effect of oral (OT) and parenteral (PT) administration of enrofloxacin to weaners on untreated contact animals. We assessed a) fluoroquinolone occurrence in the blood serum of untreated contact animals (COT, CPT); b) resistance to (fluoro)quinolones in commensal Escherichia coli (E. coli)<\/span> in OT, PT, COT and CPT compared to the control (CON), and c) resistance to other antimicrobials in E. coli<\/span> in OT, PT, COT and CPT compared to the initial situation before the treatment in these groups. Five groups of 14 weaners each were housed in three separate rooms (OT with COT, PT with CPT, CON alone). OT and PT were treated with enrofloxacin for five days. Rectal swabs and blood samples were taken before, during and until 51 days after treatment. Enro- and ciprofloxacin were detected in all treated, all COT and half of the CPT pigs. Neither through selective isolation nor by susceptibility testing of one random non-selectively isolated faecal E. coli<\/span> per sample, resistance to ciprofloxacin (metabolite of enrofloxacin) and nalidixic acid was detected in both treatment and contact groups during and short after treatment. However, a transient increase of E. coli<\/span> resistant to antimicrobials other than quinolones followed the treatment in isolates from OT and COT (e.g. ampicillin p lt; 0.05). In conclusion, animals in contact with treated animals are exposed to and can intake antimicrobials. Animals in contact with orally treated animals show occurrence of antibiotic resistant E. coli<\/span>. Further studies are needed to show whether these preliminary findings can be confirmed under different conditions and with more sensitive detection methods. <\/p>

Keywords<\/span>
Fluoroquinolone, resistance, route, transfer<\/p>

Zusammenfassung<\/span>
Ziel der Studie war, den Effekt der oralen (OT) und parenteralen (PT) Verabreichung von Enrofloxacin an Aufzuchtschweine auf unbehandelte Kontakttiere zu untersuchen. Untersucht wurde a) Fluorchinolonpr\u00e4senz im Blutserum von unbehandelten Kontakttieren (COT, CPT); b) Resistenz gegen (Fluor-)Chinolone in kommensalen Escherichia coli (E. coli)<\/span> in OT, PT, COT und CPT im Vergleich zur Kontrollgruppe (CON) und c) Resistenz gegen\u00fcber anderen Antibiotika in E. coli<\/span> in OT, PT, COT und CPT im Vergleich zur Ausgangssituation vor der Behandlung in diesen Gruppen. F\u00fcnf Gruppen von jeweils 14 Absetzern wurden in drei getrennten R\u00e4umen untergebracht (OT mit COT, PT mit CPT, CON alleine). OT und PT wurden mit Enrofloxacin f\u00fcr f\u00fcnf Tage behandelt. Rektaltupfer und Blutproben wurden vor, w\u00e4hrend und bis 51 Tage nach der Behandlung genommen. Enround Ciprofloxacin wurden in allen behandelten, allen COT und der H\u00e4lfte der CPT Schweine nachgewiesen. Es wurde keine Resistenz gegen\u00fcber Ciprofloxacin (Metabolit von Enrofloxacin) und Nalidixins\u00e4ure in beiden Behandlungs- und ihren Kontaktgruppen w\u00e4hrend und kurz nach der Behandlung nachgewiesen \u2013 weder durch selektive Isolierung noch durch Sensibilit\u00e4tstestung eines zuf\u00e4llig, nicht-selektiv isolierten f\u00e4kalen E. coli<\/span> pro Probe. Allerdings folgte auf die Behandlung ein vor\u00fcbergehender Anstieg resistenter E. coli<\/span> gegen\u00fcber anderen Antibiotika als Chinolonen in den Isolaten aus OT und COT (z. B. Ampicillin, p lt; 0,05). Wir schlussfolgern, dass die Kontakttiere behandelter Tiere Antibiotika ausgesetzt sind und diese aufnehmen und Kontakttiere oral behandelter Tiere antibiotisch resistente E. coli<\/span> aufweisen k\u00f6nnen. Weitere Untersuchungen sind notwendig, um zu zeigen, ob diese ersten Ergebnisse unter anderen Bedingungen und mit erweiterten Nachweismethoden best\u00e4tigt werden k\u00f6nnen.<\/p>

Schl\u00fcsselw\u00f6rter<\/span>
Fluorchinolon, Resistenz, Verabreichungsweg, \u00dcbertragung<\/p>","categories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"],"fromDate":"Mar 27, 2018 11:00:36 AM","oldUrls":["http:\/\/vetline.de\/antimicrobial-susceptibility-in-faecal-escherichia-coli-from-pigs-after-enrofloxacin-administration-in-an-experimental-environment\/150\/3216\/107369","http:\/\/vetline.de\/antimicrobial-susceptibility-in-faecal-escherichia-coli-from-pigs-after-enrofloxacin-administration-in-an-experimental-environment\/150\/3130\/107369"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl M\u00fcnch Tier\u00e4rztl Wochensch","doiDocumentUri":"https:\/\/vetline.de\/files\/smfiledata\/7\/1\/0\/9\/8\/1\/BMW_AOP_17079_onl300.pdf","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochensch","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-17079","doiFirstPage":".","doiLastPage":"..","doiTransmitted":true,"doiAuthor":"Burow E, Grobbel M, Tenhagen B-A, Simoneit C, Ladwig M, Szab\u00f3 I, Wendt D, Banneke S, K\u00e4sbohrer A","pdf":{"path":"http:\/\/data\/BMW_AOP_17079_onl300.pdf","title":"BMTW_OA_17079_Burow","description":"Antimicrobial susceptibility in faecal Escherichia coli from pigs after enrofloxacin administration in an experimental environment"},"authors":[{"firstName":"E","middleName":"","lastName":"Burow"},{"firstName":"M","middleName":"","lastName":"Grobbel"},{"firstName":"B","middleName":"A","lastName":"Tenhagen"},{"firstName":"C","middleName":"","lastName":"Simoneit"},{"firstName":"M","middleName":"","lastName":"Ladwig"},{"firstName":"I","middleName":"","lastName":"Szab\u00f3"},{"firstName":"D","middleName":"","lastName":"Wendt"},{"firstName":"S","middleName":"","lastName":"Banneke"},{"firstName":"A","middleName":"","lastName":"K\u00e4sbohrer"}],"contentOptimised":"

Summary<\/strong>
The study objective was to evaluate the effect of oral (OT) and parenteral (PT) administration of enrofloxacin to weaners on untreated contact animals. We assessed a) fluoroquinolone occurrence in the blood serum of untreated contact animals (COT, CPT); b) resistance to (fluoro)quinolones in commensal Escherichia coli (E. coli)<\/em> in OT, PT, COT and CPT compared to the control (CON), and c) resistance to other antimicrobials in E. coli<\/em> in OT, PT, COT and CPT compared to the initial situation before the treatment in these groups. Five groups of 14 weaners each were housed in three separate rooms (OT with COT, PT with CPT, CON alone). OT and PT were treated with enrofloxacin for five days. Rectal swabs and blood samples were taken before, during and until 51 days after treatment. Enro- and ciprofloxacin were detected in all treated, all COT and half of the CPT pigs. Neither through selective isolation nor by susceptibility testing of one random non-selectively isolated faecal E. coli<\/em> per sample, resistance to ciprofloxacin (metabolite of enrofloxacin) and nalidixic acid was detected in both treatment and contact groups during and short after treatment. However, a transient increase of E. coli<\/em> resistant to antimicrobials other than quinolones followed the treatment in isolates from OT and COT (e.g. ampicillin p lt; 0.05). In conclusion, animals in contact with treated animals are exposed to and can intake antimicrobials. Animals in contact with orally treated animals show occurrence of antibiotic resistant E. coli<\/em>. Further studies are needed to show whether these preliminary findings can be confirmed under different conditions and with more sensitive detection methods. <\/p>

Keywords:<\/strong>
Fluoroquinolone, resistance, route, transfer<\/p>

Zusammenfassung<\/strong>
Ziel der Studie war, den Effekt der oralen (OT) und parenteralen (PT) Verabreichung von Enrofloxacin an Aufzuchtschweine auf unbehandelte Kontakttiere zu untersuchen. Untersucht wurde a) Fluorchinolonpr\u00e4senz im Blutserum von unbehandelten Kontakttieren (COT, CPT); b) Resistenz gegen (Fluor-)Chinolone in kommensalen Escherichia coli (E. coli)<\/em> in OT, PT, COT und CPT im Vergleich zur Kontrollgruppe (CON) und c) Resistenz gegen\u00fcber anderen Antibiotika in E. coli<\/em> in OT, PT, COT und CPT im Vergleich zur Ausgangssituation vor der Behandlung in diesen Gruppen. F\u00fcnf Gruppen von jeweils 14 Absetzern wurden in drei getrennten R\u00e4umen untergebracht (OT mit COT, PT mit CPT, CON alleine). OT und PT wurden mit Enrofloxacin f\u00fcr f\u00fcnf Tage behandelt. Rektaltupfer und Blutproben wurden vor, w\u00e4hrend und bis 51 Tage nach der Behandlung genommen. Enround Ciprofloxacin wurden in allen behandelten, allen COT und der H\u00e4lfte der CPT Schweine nachgewiesen. Es wurde keine Resistenz gegen\u00fcber Ciprofloxacin (Metabolit von Enrofloxacin) und Nalidixins\u00e4ure in beiden Behandlungs- und ihren Kontaktgruppen w\u00e4hrend und kurz nach der Behandlung nachgewiesen \u2013 weder durch selektive Isolierung noch durch Sensibilit\u00e4tstestung eines zuf\u00e4llig, nicht-selektiv isolierten f\u00e4kalen E. coli<\/em> pro Probe. Allerdings folgte auf die Behandlung ein vor\u00fcbergehender Anstieg resistenter E. coli<\/em> gegen\u00fcber anderen Antibiotika als Chinolonen in den Isolaten aus OT und COT (z. B. Ampicillin, p lt; 0,05). Wir schlussfolgern, dass die Kontakttiere behandelter Tiere Antibiotika ausgesetzt sind und diese aufnehmen und Kontakttiere oral behandelter Tiere antibiotisch resistente E. coli<\/em> aufweisen k\u00f6nnen. Weitere Untersuchungen sind notwendig, um zu zeigen, ob diese ersten Ergebnisse unter anderen Bedingungen und mit erweiterten Nachweismethoden best\u00e4tigt werden k\u00f6nnen.<\/p>

Schl\u00fcsselw\u00f6rter:<\/strong>
Fluorchinolon, Resistenz, Verabreichungsweg, \u00dcbertragung<\/p>","primaryLanguage":"englisch","summary":"The study objective was to evaluate the effect of oral (OT) and parenteral (PT) administration of enrofloxacin to weaners on untreated contact animals. We assessed a) fluoroquinolone occurrence in the blood serum of untreated contact animals (COT, CPT); b) resistance to (fluoro)quinolones in commensal Escherichia coli (E. coli)<\/em> in OT, PT, COT and CPT compared to the control (CON), and c) resistance to other antimicrobials in E. coli<\/em> in OT, PT, COT and CPT compared to the initial situation before the treatment in these groups. Five groups of 14 weaners each were housed in three separate rooms (OT with COT, PT with CPT, CON alone). OT and PT were treated with enrofloxacin for five days. Rectal swabs and blood samples were taken before, during and until 51 days after treatment. Enro- and ciprofloxacin were detected in all treated, all COT and half of the CPT pigs. Neither through selective isolation nor by susceptibility testing of one random non-selectively isolated faecal E. coli<\/em> per sample, resistance to ciprofloxacin (metabolite of enrofloxacin) and nalidixic acid was detected in both treatment and contact groups during and short after treatment. However, a transient increase of E. coli<\/em> resistant to antimicrobials other than quinolones followed the treatment in isolates from OT and COT (e.g. ampicillin p lt; 0.05). In conclusion, animals in contact with treated animals are exposed to and can intake antimicrobials. Animals in contact with orally treated animals show occurrence of antibiotic resistant E. coli<\/em>. Further studies are needed to show whether these preliminary findings can be confirmed under different conditions and with more sensitive detection methods. <\/p>

","keywords":["Fluoroquinolone","resistance","route","transfer"],"zusammenfassung":"Ziel der Studie war, den Effekt der oralen (OT) und parenteralen (PT) Verabreichung von Enrofloxacin an Aufzuchtschweine auf unbehandelte Kontakttiere zu untersuchen. Untersucht wurde a) Fluorchinolonpr\u00e4senz im Blutserum von unbehandelten Kontakttieren (COT, CPT); b) Resistenz gegen (Fluor-)Chinolone in kommensalen Escherichia coli (E. coli)<\/em> in OT, PT, COT und CPT im Vergleich zur Kontrollgruppe (CON) und c) Resistenz gegen\u00fcber anderen Antibiotika in E. coli<\/em> in OT, PT, COT und CPT im Vergleich zur Ausgangssituation vor der Behandlung in diesen Gruppen. F\u00fcnf Gruppen von jeweils 14 Absetzern wurden in drei getrennten R\u00e4umen untergebracht (OT mit COT, PT mit CPT, CON alleine). OT und PT wurden mit Enrofloxacin f\u00fcr f\u00fcnf Tage behandelt. Rektaltupfer und Blutproben wurden vor, w\u00e4hrend und bis 51 Tage nach der Behandlung genommen. Enround Ciprofloxacin wurden in allen behandelten, allen COT und der H\u00e4lfte der CPT Schweine nachgewiesen. Es wurde keine Resistenz gegen\u00fcber Ciprofloxacin (Metabolit von Enrofloxacin) und Nalidixins\u00e4ure in beiden Behandlungs- und ihren Kontaktgruppen w\u00e4hrend und kurz nach der Behandlung nachgewiesen \u2013 weder durch selektive Isolierung noch durch Sensibilit\u00e4tstestung eines zuf\u00e4llig, nicht-selektiv isolierten f\u00e4kalen E. coli<\/em> pro Probe. Allerdings folgte auf die Behandlung ein vor\u00fcbergehender Anstieg resistenter E. coli<\/em> gegen\u00fcber anderen Antibiotika als Chinolonen in den Isolaten aus OT und COT (z. B. Ampicillin, p lt; 0,05). Wir schlussfolgern, dass die Kontakttiere behandelter Tiere Antibiotika ausgesetzt sind und diese aufnehmen und Kontakttiere oral behandelter Tiere antibiotisch resistente E. coli<\/em> aufweisen k\u00f6nnen. Weitere Untersuchungen sind notwendig, um zu zeigen, ob diese ersten Ergebnisse unter anderen Bedingungen und mit erweiterten Nachweismethoden best\u00e4tigt werden k\u00f6nnen.<\/p>

","schluesselwoerter":["Fluorchinolon","Resistenz","Verabreichungsweg","\u00dcbertragung"],"translatedTitle":"Empfindlichkeit f\u00e4kaler Escherichia coli von Schweinen gegen\u00fcber Antibiotika nach Enrofloxacinverabreichung in einer experimentellen Umgebung","abstractE":"The study objective was to evaluate the effect of oral (OT) and parenteral (PT) administration of enrofloxacin to weaners on untreated contact animals. We assessed a) fluoroquinolone occurrence in the blood serum of untreated contact animals (COT, CPT); b) resistance to (fluoro)quinolones in commensal Escherichia coli (E. coli) in OT, PT, COT and CPT compared to the control (CON), and c) resistance to other antimicrobials in E. coli in OT, PT, COT and CPT compared to the initial situation before the treatment in these groups. Five groups of 14 weaners each were housed in three separate rooms (OT with COT, PT with CPT, CON alone). OT and PT were treated with enrofloxacin for five days. Rectal swabs and blood samples were taken before, during and until 51 days after treatment. Enro- and ciprofloxacin were detected in all treated, all COT and half of the CPT pigs. Neither through selective isolation nor by susceptibility testing of one random non-selectively isolated faecal E. coli per sample, resistance to ciprofloxacin (metabolite of enrofloxacin) and nalidixic acid was detected in both treatment and contact groups during and short after treatment. However, a transient increase of E. coli resistant to antimicrobials other than quinolones followed the treatment in isolates from OT and COT (e.g. ampicillin p lt; 0.05). In conclusion, animals in contact with treated animals are exposed to and can intake antimicrobials. Animals in contact with orally treated animals show occurrence of antibiotic resistant E. coli. Further studies are needed to show whether these preliminary findings can be confirmed under different conditions and with more sensitive detection methods. ","date":{"year":2018,"date":"03\/2018","accepted":"2018-03-27"},"volume":131,"openAccess":true,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"","redirects":["antimicrobial-susceptibility-in-faecal-escherichia-coli-from-pigs-after-enrofloxacin-administration-in-an-experimental-environment\/150\/3216\/107369","antimicrobial-susceptibility-in-faecal-escherichia-coli-from-pigs-after-enrofloxacin-administration-in-an-experimental-environment\/150\/3130\/107369"],"tierartCategories":[],"artikelartCategories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"]} CY - Hannover DA - 03/2018 DO - 10.2376/0005-9366-17079 LA - English N2 - The study objective was to evaluate the effect of oral (OT) and parenteral (PT) administration of enrofloxacin to weaners on untreated contact animals. We assessed a) fluoroquinolone occurrence in the blood serum of untreated contact animals (COT, CPT); b) resistance to (fluoro)quinolones in commensal Escherichia coli (E. coli) in OT, PT, COT and CPT compared to the control (CON), and c) resistance to other antimicrobials in E. coli in OT, PT, COT and CPT compared to the initial situation before the treatment in these groups. Five groups of 14 weaners each were housed in three separate rooms (OT with COT, PT with CPT, CON alone). OT and PT were treated with enrofloxacin for five days. Rectal swabs and blood samples were taken before, during and until 51 days after treatment. Enro- and ciprofloxacin were detected in all treated, all COT and half of the CPT pigs. Neither through selective isolation nor by susceptibility testing of one random non-selectively isolated faecal E. coli per sample, resistance to ciprofloxacin (metabolite of enrofloxacin) and nalidixic acid was detected in both treatment and contact groups during and short after treatment. However, a transient increase of E. coli resistant to antimicrobials other than quinolones followed the treatment in isolates from OT and COT (e.g. ampicillin p lt; 0.05). In conclusion, animals in contact with treated animals are exposed to and can intake antimicrobials. Animals in contact with orally treated animals show occurrence of antibiotic resistant E. coli. Further studies are needed to show whether these preliminary findings can be confirmed under different conditions and with more sensitive detection methods. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2018 T1 - Antimicrobial susceptibility in faecal Escherichia coli from pigs after enrofloxacin administration in an experimental environment T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Antimicrobial susceptibility in faecal Escherichia coli from pigs after enrofloxacin administration in an experimental environment TT - Empfindlichkeit fäkaler Escherichia coli von Schweinen gegenüber Antibiotika nach Enrofloxacinverabreichung in einer experimentellen Umgebung VL - 131 SN - 0005-9366 ER -