TY - JOUR KW - colic KW - gut ischemia KW - epithelial alteration KW - alcohol dehydrogenase KW - prognostic marker AU - G Köller AU - M Breitenstein AU - N Graneß AU - S Recknagel AU - R Barsnick AU - U Walliser AU - W Scheidemann AU - A Bienert-Zeit AU - D Donandt AU - G Stadtbäumer AU - M Paar AU - H Hell AU - H-A Schoon AU - G Schusser AB - The diagnosis and especially the prognosis in horses with strangulating obstructions represent a particular challenge for the veterinarian. Reliable parameters are needed to facilitate choosing the right therapy. In a study of Gomaa et al. (2011) it was found out that alcohol dehydrogenase (ADH) with an activity of gt; 80 U/L in colic horses with small intestinal strangulation indicated for non-surviving with a sensitivity of 66.7% and a specificity of 94.4%. This current study documents the ADH activity in the serum of 70 horses with small intestinal strangulation and 11 horses with strangulation of the large intestine. Samples of all strangulated intestinal segments were evaluated histologically and clinical data of the horses were collected. The aim of this study was to investigate the relationship between serum ADH activity and the histologically detectable epithelial damage of the ischemic intestine, and the duration of colic, length of the strangulated intestinal part and clinical outcome. Horses with a colic duration of ≥ 4 hours and a ≥ 300 cm strangulated small intestine had significantly increased ADH activity of 41 U/L (median) compared to normal horses (16.3 U/L) and the ROC selected a cut-off value of 25 U/L with a sensitivity of 100% and a specificity of 77%. These horses had complete loss of the villus epithelium with necrosis of the epithelial crypt cells, which indicate a resection of the gut segment involved. Serum ADH activity was below 80 U/L in all horses that survived an intestinal resection. BT - Berliner und Münchener Tierärztliche Wochenschrift C1 - {"oldId":99097,"title":"Multi-centre study on the evaluation of alcohol dehydrogenase activity in the serum of horses with intestinal strangulation","topline":"Open Access","teaserText":"Multizentrische Studie \u00fcber die Evaluierung der Alkoholdehydogenaseaktivit\u00e4t im Serum von Pferden mit Strangulationsileus","content":"

Summary<\/span>
The diagnosis and especially the prognosis in horses with strangulating obstructions represent a particular challenge for the veterinarian. Reliable parameters are needed to facilitate choosing the right therapy.
In a study of Gomaa et al. (2011) it was found out that alcohol dehydrogenase (ADH) with an activity of gt; 80 U\/L in colic horses with small intestinal strangulation indicated for non-surviving with a sensitivity of 66.7% and a specificity of 94.4%. This current study documents the ADH activity in the serum of 70 horses with small intestinal strangulation and 11 horses with strangulation of the large intestine. Samples of all strangulated intestinal segments were evaluated histologically and clinical data of the horses were collected. The aim of this study was to investigate the relationship between serum ADH activity and the histologically detectable epithelial damage of the ischemic intestine, and the duration of colic, length of the strangulated intestinal part and clinical outcome. Horses with a colic duration of \u2265 4 hours and a \u2265 300 cm strangulated small intestine had significantly increased ADH activity of 41 U\/L (median) compared to normal horses (16.3 U\/L) and the ROC selected a cut-off value of 25 U\/L with a sensitivity of 100% and a specificity of 77%. These horses had complete loss of the villus epithelium with necrosis of the epithelial crypt cells, which indicate a resection of the gut segment involved. Serum ADH activity was below 80 U\/L in all horses that survived an intestinal resection.

Keywords:<\/span> colic, gut ischemia, epithelial alteration, alcohol dehydrogenase, prognostic marker<\/p>

Zusammenfassung<\/span>
Die Diagnose und speziell die Prognose f\u00fcr ein Pferd mit einem Strangulationsileus ist eine besondere Herausforderung f\u00fcr den Tierarzt, wobei Marker erforderlich sind, um die richtige Therapie zu w\u00e4hlen. Dabei erwies sich in der Studie von Gomaa et al. (2011), dass die Alkoholdehydrogenase (ADH) mit einer Aktivit\u00e4t gt; 80 U\/L bei Kolikpferden mit einem Strangulationsileus auf ein Nicht-\u00dcberleben mit einer Sensitivit\u00e4t von 66,7 % und eine Spezifit\u00e4t von 94,4 % hinweist. In dieser Studie wurden die ADH-Aktivit\u00e4ten von 70 Pferden mit D\u00fcnndarm- und von 11 Pferden mit Kolonstrangulation gemessen. Gewebeproben von den strangulierten Darmsegmenten wurden entnommen und die Epithelalteration histologisch beurteilt und die Kolikdauer sowie die L\u00e4nge des strangulierten Darmteiles dokumentiert. Das Ziel dieser Arbeit war es, den Zusammenhang zwischen ADH-Aktivit\u00e4t und Epithelalteration des isch\u00e4mischen Darmteiles zu eruieren. Pferde mit einer Kolikdauer \u2265 4 Stunden und einer L\u00e4nge des strangulierten Darmteiles \u2265 300 cm hatten signifikant h\u00f6here ADH-Aktivit\u00e4ten (41 U\/L, median) in Vergleich zu Normalpferden (16,3 U\/L, median). Diese Pferde hatten einen kompletten Verlust der Epithelzellen der Villi mit nekrotischen Kryptepithelzellen, der auf eine Resektion des involvierten Darmteiles hinwies, wobei ein cut-off-Wert von 25 U\/L bei einer Sensitivit\u00e4t von 100 % und einer Spezifit\u00e4t von 77 % ermittelt werden konnte. Die Serum-ADH-Aktivit\u00e4ten lagen unter 80 U\/L bei den Pferden, die mit D\u00fcnndarmresektion \u00fcberlebten.

Schl\u00fcsselw\u00f6rter:<\/span> Kolik, Darmisch\u00e4mie, Epithelalteration, Alkoholdehydrogenase, Prognosemarker

<\/p>","categories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"],"fromDate":"Nov 17, 2016 10:14:07 AM","oldUrls":["http:\/\/vetline.de\/multi-centre-study-on-the-evaluation-of-alcohol-dehydrogenase-activity-in-the-serum-of-horses-with-intestinal-strangulation\/150\/3216\/99097","http:\/\/vetline.de\/multi-centre-study-on-the-evaluation-of-alcohol-dehydrogenase-activity-in-the-serum-of-horses-with-intestinal-strangulation\/150\/3130\/99097"],"doiLanguage":"englisch","doiProductFormat":"online","doiPublisher":"Schl\u00fctersche Verlagsgesellschaft mbH & Co. KG","doiSerialWorkTitle":"Berl M\u00fcnch Tier\u00e4rztl Wochensch","doiDocumentUri":"http:\/\/cf01.vetline.schluetersche.de\/files\/smfiledata\/6\/1\/6\/3\/4\/3\/BMW_OA_16074_Schusser_onl300.pdf","doiSource":"Berl M\u00fcnch Tier\u00e4rztl Wochensch","doiissn":"0005-9366","doiNr":"10.2376\/0005-9366-16074","doiFirstPage":".","doiLastPage":"..","doiTransmitted":true,"doiAuthor":"K\u00f6ller G, Breitenstein M, Grane\u00df N, Recknagel S, Barsnick R, Walliser U, Scheidemann W, Bienert-Zeit A, Donandt D, Stadtb\u00e4umer G, Paar M, Hell H, Schoon H-A, Schusser GF","pdf":{"path":"http:\/\/data\/BMW_OA_16074_Schusser_onl300.pdf","title":"BMTW OA 16074 Schusser","description":""},"authors":[{"firstName":"G","middleName":"","lastName":"K\u00f6ller"},{"firstName":"M","middleName":"","lastName":"Breitenstein"},{"firstName":"N","middleName":"","lastName":"Grane\u00df"},{"firstName":"S","middleName":"","lastName":"Recknagel"},{"firstName":"R","middleName":"","lastName":"Barsnick"},{"firstName":"U","middleName":"","lastName":"Walliser"},{"firstName":"W","middleName":"","lastName":"Scheidemann"},{"firstName":"A","middleName":"","lastName":"Bienert-Zeit"},{"firstName":"D","middleName":"","lastName":"Donandt"},{"firstName":"G","middleName":"","lastName":"Stadtb\u00e4umer"},{"firstName":"M","middleName":"","lastName":"Paar"},{"firstName":"H","middleName":"","lastName":"Hell"},{"firstName":"H","middleName":"A","lastName":"Schoon"},{"firstName":"G","middleName":"F","lastName":"Schusser"}],"contentOptimised":"

Summary<\/strong>
The diagnosis and especially the prognosis in horses with strangulating obstructions represent a particular challenge for the veterinarian. Reliable parameters are needed to facilitate choosing the right therapy.
In a study of Gomaa et al. (2011) it was found out that alcohol dehydrogenase (ADH) with an activity of gt; 80 U\/L in colic horses with small intestinal strangulation indicated for non-surviving with a sensitivity of 66.7% and a specificity of 94.4%. This current study documents the ADH activity in the serum of 70 horses with small intestinal strangulation and 11 horses with strangulation of the large intestine. Samples of all strangulated intestinal segments were evaluated histologically and clinical data of the horses were collected. The aim of this study was to investigate the relationship between serum ADH activity and the histologically detectable epithelial damage of the ischemic intestine, and the duration of colic, length of the strangulated intestinal part and clinical outcome. Horses with a colic duration of \u2265 4 hours and a \u2265 300 cm strangulated small intestine had significantly increased ADH activity of 41 U\/L (median) compared to normal horses (16.3 U\/L) and the ROC selected a cut-off value of 25 U\/L with a sensitivity of 100% and a specificity of 77%. These horses had complete loss of the villus epithelium with necrosis of the epithelial crypt cells, which indicate a resection of the gut segment involved. Serum ADH activity was below 80 U\/L in all horses that survived an intestinal resection.

Keywords:<\/strong> colic, gut ischemia, epithelial alteration, alcohol dehydrogenase, prognostic marker<\/p>

Zusammenfassung<\/strong>
Die Diagnose und speziell die Prognose f\u00fcr ein Pferd mit einem Strangulationsileus ist eine besondere Herausforderung f\u00fcr den Tierarzt, wobei Marker erforderlich sind, um die richtige Therapie zu w\u00e4hlen. Dabei erwies sich in der Studie von Gomaa et al. (2011), dass die Alkoholdehydrogenase (ADH) mit einer Aktivit\u00e4t gt; 80 U\/L bei Kolikpferden mit einem Strangulationsileus auf ein Nicht-\u00dcberleben mit einer Sensitivit\u00e4t von 66,7 % und eine Spezifit\u00e4t von 94,4 % hinweist. In dieser Studie wurden die ADH-Aktivit\u00e4ten von 70 Pferden mit D\u00fcnndarm- und von 11 Pferden mit Kolonstrangulation gemessen. Gewebeproben von den strangulierten Darmsegmenten wurden entnommen und die Epithelalteration histologisch beurteilt und die Kolikdauer sowie die L\u00e4nge des strangulierten Darmteiles dokumentiert. Das Ziel dieser Arbeit war es, den Zusammenhang zwischen ADH-Aktivit\u00e4t und Epithelalteration des isch\u00e4mischen Darmteiles zu eruieren. Pferde mit einer Kolikdauer \u2265 4 Stunden und einer L\u00e4nge des strangulierten Darmteiles \u2265 300 cm hatten signifikant h\u00f6here ADH-Aktivit\u00e4ten (41 U\/L, median) in Vergleich zu Normalpferden (16,3 U\/L, median). Diese Pferde hatten einen kompletten Verlust der Epithelzellen der Villi mit nekrotischen Kryptepithelzellen, der auf eine Resektion des involvierten Darmteiles hinwies, wobei ein cut-off-Wert von 25 U\/L bei einer Sensitivit\u00e4t von 100 % und einer Spezifit\u00e4t von 77 % ermittelt werden konnte. Die Serum-ADH-Aktivit\u00e4ten lagen unter 80 U\/L bei den Pferden, die mit D\u00fcnndarmresektion \u00fcberlebten.

Schl\u00fcsselw\u00f6rter:<\/strong> Kolik, Darmisch\u00e4mie, Epithelalteration, Alkoholdehydrogenase, Prognosemarker

<\/p>","primaryLanguage":"englisch","summary":"The diagnosis and especially the prognosis in horses with strangulating obstructions represent a particular challenge for the veterinarian. Reliable parameters are needed to facilitate choosing the right therapy.
In a study of Gomaa et al. (2011) it was found out that alcohol dehydrogenase (ADH) with an activity of gt; 80 U\/L in colic horses with small intestinal strangulation indicated for non-surviving with a sensitivity of 66.7% and a specificity of 94.4%. This current study documents the ADH activity in the serum of 70 horses with small intestinal strangulation and 11 horses with strangulation of the large intestine. Samples of all strangulated intestinal segments were evaluated histologically and clinical data of the horses were collected. The aim of this study was to investigate the relationship between serum ADH activity and the histologically detectable epithelial damage of the ischemic intestine, and the duration of colic, length of the strangulated intestinal part and clinical outcome. Horses with a colic duration of \u2265 4 hours and a \u2265 300 cm strangulated small intestine had significantly increased ADH activity of 41 U\/L (median) compared to normal horses (16.3 U\/L) and the ROC selected a cut-off value of 25 U\/L with a sensitivity of 100% and a specificity of 77%. These horses had complete loss of the villus epithelium with necrosis of the epithelial crypt cells, which indicate a resection of the gut segment involved. Serum ADH activity was below 80 U\/L in all horses that survived an intestinal resection.","keywords":["colic","gut ischemia","epithelial alteration","alcohol dehydrogenase","prognostic marker"],"zusammenfassung":"Die Diagnose und speziell die Prognose f\u00fcr ein Pferd mit einem Strangulationsileus ist eine besondere Herausforderung f\u00fcr den Tierarzt, wobei Marker erforderlich sind, um die richtige Therapie zu w\u00e4hlen. Dabei erwies sich in der Studie von Gomaa et al. (2011), dass die Alkoholdehydrogenase (ADH) mit einer Aktivit\u00e4t gt; 80 U\/L bei Kolikpferden mit einem Strangulationsileus auf ein Nicht-\u00dcberleben mit einer Sensitivit\u00e4t von 66,7 % und eine Spezifit\u00e4t von 94,4 % hinweist. In dieser Studie wurden die ADH-Aktivit\u00e4ten von 70 Pferden mit D\u00fcnndarm- und von 11 Pferden mit Kolonstrangulation gemessen. Gewebeproben von den strangulierten Darmsegmenten wurden entnommen und die Epithelalteration histologisch beurteilt und die Kolikdauer sowie die L\u00e4nge des strangulierten Darmteiles dokumentiert. Das Ziel dieser Arbeit war es, den Zusammenhang zwischen ADH-Aktivit\u00e4t und Epithelalteration des isch\u00e4mischen Darmteiles zu eruieren. Pferde mit einer Kolikdauer \u2265 4 Stunden und einer L\u00e4nge des strangulierten Darmteiles \u2265 300 cm hatten signifikant h\u00f6here ADH-Aktivit\u00e4ten (41 U\/L, median) in Vergleich zu Normalpferden (16,3 U\/L, median). Diese Pferde hatten einen kompletten Verlust der Epithelzellen der Villi mit nekrotischen Kryptepithelzellen, der auf eine Resektion des involvierten Darmteiles hinwies, wobei ein cut-off-Wert von 25 U\/L bei einer Sensitivit\u00e4t von 100 % und einer Spezifit\u00e4t von 77 % ermittelt werden konnte. Die Serum-ADH-Aktivit\u00e4ten lagen unter 80 U\/L bei den Pferden, die mit D\u00fcnndarmresektion \u00fcberlebten.","schluesselwoerter":["Kolik","Darmisch\u00e4mie","Epithelalteration","Alkoholdehydrogenase","Prognosemarker"],"translatedTitle":"Multizentrische Studie \u00fcber die Evaluierung der Alkoholdehydogenaseaktivit\u00e4t im Serum von Pferden mit Strangulationsileus","abstractE":"The diagnosis and especially the prognosis in horses with strangulating obstructions represent a particular challenge for the veterinarian. Reliable parameters are needed to facilitate choosing the right therapy. In a study of Gomaa et al. (2011) it was found out that alcohol dehydrogenase (ADH) with an activity of gt; 80 U\/L in colic horses with small intestinal strangulation indicated for non-surviving with a sensitivity of 66.7% and a specificity of 94.4%. This current study documents the ADH activity in the serum of 70 horses with small intestinal strangulation and 11 horses with strangulation of the large intestine. Samples of all strangulated intestinal segments were evaluated histologically and clinical data of the horses were collected. The aim of this study was to investigate the relationship between serum ADH activity and the histologically detectable epithelial damage of the ischemic intestine, and the duration of colic, length of the strangulated intestinal part and clinical outcome. Horses with a colic duration of \u2265 4 hours and a \u2265 300 cm strangulated small intestine had significantly increased ADH activity of 41 U\/L (median) compared to normal horses (16.3 U\/L) and the ROC selected a cut-off value of 25 U\/L with a sensitivity of 100% and a specificity of 77%. These horses had complete loss of the villus epithelium with necrosis of the epithelial crypt cells, which indicate a resection of the gut segment involved. Serum ADH activity was below 80 U\/L in all horses that survived an intestinal resection.","date":{"year":2016,"date":"11\/2016","accepted":"2016-11-17"},"volume":129,"openAccess":true,"journal":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift","titleImageId":944,"pages":"","redirects":["multi-centre-study-on-the-evaluation-of-alcohol-dehydrogenase-activity-in-the-serum-of-horses-with-intestinal-strangulation\/150\/3216\/99097","multi-centre-study-on-the-evaluation-of-alcohol-dehydrogenase-activity-in-the-serum-of-horses-with-intestinal-strangulation\/150\/3130\/99097"],"tierartCategories":[],"artikelartCategories":["Open Access","Tier\u00e4rztliche Wochenschrift","Abostufe BMTW","Fachartikel","Abostufe frei"]} CY - Hannover DA - 11/2016 DO - 10.2376/0005-9366-16074 LA - English N2 - The diagnosis and especially the prognosis in horses with strangulating obstructions represent a particular challenge for the veterinarian. Reliable parameters are needed to facilitate choosing the right therapy. In a study of Gomaa et al. (2011) it was found out that alcohol dehydrogenase (ADH) with an activity of gt; 80 U/L in colic horses with small intestinal strangulation indicated for non-surviving with a sensitivity of 66.7% and a specificity of 94.4%. This current study documents the ADH activity in the serum of 70 horses with small intestinal strangulation and 11 horses with strangulation of the large intestine. Samples of all strangulated intestinal segments were evaluated histologically and clinical data of the horses were collected. The aim of this study was to investigate the relationship between serum ADH activity and the histologically detectable epithelial damage of the ischemic intestine, and the duration of colic, length of the strangulated intestinal part and clinical outcome. Horses with a colic duration of ≥ 4 hours and a ≥ 300 cm strangulated small intestine had significantly increased ADH activity of 41 U/L (median) compared to normal horses (16.3 U/L) and the ROC selected a cut-off value of 25 U/L with a sensitivity of 100% and a specificity of 77%. These horses had complete loss of the villus epithelium with necrosis of the epithelial crypt cells, which indicate a resection of the gut segment involved. Serum ADH activity was below 80 U/L in all horses that survived an intestinal resection. PB - Schlütersche Verlagsgesellschaft mbH & Co. KG PP - Hannover PY - 2016 T1 - Multi-centre study on the evaluation of alcohol dehydrogenase activity in the serum of horses with intestinal strangulation T2 - Berliner und Münchener Tierärztliche Wochenschrift TI - Multi-centre study on the evaluation of alcohol dehydrogenase activity in the serum of horses with intestinal strangulation TT - Multizentrische Studie über die Evaluierung der Alkoholdehydogenaseaktivität im Serum von Pferden mit Strangulationsileus VL - 129 SN - 0005-9366 ER -