01794nas a2200289   4500000000100000000000100001008004100002260007000043653001800113653004600131653001300177653000900190653001300199100001500212700001300227700001000240700001300250700001300263700001500276700002600291700001300317700001900330245012800349490000800477520100500485022001401490       2020                            d  c03/2020bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover10a16S rRNA gene10a16S-23S rDNA intergenic spacer region ISR10agene tuf10arpoB10apyolysin1 aM Alssahen1 aM Peters1 aJ Rau1 aA Hassan1 aO Sammra1 aC Lämmler1 aE Prenger-Berninghoff1 aM Plötz1 aA Abdulmawjood00aPhenotypic and genotypic approach  to characterize a Trueperella pyogenes strain isolated from an Eurasian lynx (Lynx lynx)0 v1333 aIn the present study, a single Trueperella (T.) pyogenes strain isolated from a pneumonic lung of an Eurasian lynx (Lynx lynx) was identified and further characterized by phenotypical investigations, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), by Fourier Transform Infrared Spectroscopy (FT-IR) and genotypically by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay and by sequencing the 16S ribosomal RNA (rRNA) gene, the 16S-23S rDNA intergenic spacer region (ISR), the elongation factor tu encoding gene tuf, the β-subunit of bacterial RNA polymerase encoding gene rpoB and by sequencing the virulence factor pyolysin encoding gene plo. 
The present study gives a detailed characterization of a single strain of the species T. pyogenes from an Eurasian lynx. However, the route of infection of the Eurasian lynx with the bacterial pathogen remains unclear.  a0005-9366