01834nas a2200277   4500000000100000000000100001008004100002260007000043653003000113653002400143653000800167653001500175100001600190700001500206700001500221700001700236700001400253700001400267700001700281245011600298250000800414300001200422490000800434520110000442022001401542       2015                            d  c07/2015bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover10aAnaplasma phagocytophilum10atick-borne diseases10aPCR10awild bison1 aB Dzięgiel1 aŁ Adaszek1 aM Krzysiak1 aM Skrzypczak1 aM Adaszek1 aB Furmaga1 aS Winiarczyk00aThe occurrence of Anaplasma phagocytophilum in wild bison from the Bialowieza Primeval Forest in Eastern Poland  a7/8  a310-3140 v1283 aThe aim of the present study was to investigate the occurrence of Anaplasma spp. in a group of 120 wild bison (Bison bonasus) from the Bialowieza Primeval Forest in eastern Poland and to determine which species of Anaplasma could infect these animals based on a PCR of a part of the 16S rRNA gene followed by sequencing. The PCR technique showed the presence of 16S rRNA Anaplasma spp. genetic material in the blood of 22 from a total of 120 animals. DNA amplification by means of the primers EHR 521 and EHR 747 gave a product size of 252-bp. The sequences of the PCR products obtained showed 100% homology with each other and 100% homology with the Anaplasma phagocytophilum GU 18908 sequence from our earlier study, isolated from a horse with a clinical case of anaplasmosis. The similarity of the sequences with the sequences of the 16S rRNA gene isolated from various Anaplasma species deposited in the GeneBank, ranged between 95.8% and 98.8%. Based on the results of molecular analysis, bacterial DNA detected in the blood of 22 wild bison was identified as Anaplasma phagocytophilum.   a0005-9366