01934nas a2200253   4500000000100000000000100001008004100002260007000043653002400113653001500137653002700152653000800179100001300187700001600200700001200216700001400228700001500242245010000257250000800357300001200365490000800377520128100385022001401666       2015                            d  c07/2015bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover10arespiratory disease10aMycoplasma10abronchoalveolar lavage10aPCR1 aB Schulz1 aK Raufeisen1 aK Weber1 aS Laberke1 aK Hartmann00aComparison of the prevalence of Mycoplasma species in dogs with and without respiratory disease  a7/8  a304-3090 v1283 aAim of the study was to investigate the prevalence of Mycoplasma species in dogs with and without signs of respiratory disease. Bronchoalveolarlavage fluid (BALF) and pharyngeal swabs were collected from 29 dogs with respiratory diseases (RD) and 16 dogs without signs of RD that were euthanised because of other diseases. Samples were tested for Mycoplasma species by PCR and culture, and sequencing was performed in Mycoplasma species-positive BALF samples. Pharyngeal swabs were positive for Mycoplasma species by PCR in 91.7% of dogs with RD and 86.7% of dogs without signs of RD (p = 1.000); BALF samples were PCRpositive in 7.9% of dogs with RD and 18.8% of dogs without signs of RD (p = 0.190). Mycoplasma culture of BALF was positive in 28.6% of dogs with RD and in 18.8% without signs of RD (p = 0.70). When culture and PCR were compared, there was no significant difference in the detection rate of Mycoplasma species (p = 0.658) Sequencing detected different Mycoplasma species. Out of these, however, Mycoplasma cynos was isolated from four dogs with RD. There is no significant difference in the prevalence of Mycoplasma species between dogs with RD and dogs without evidence of RD; however, Mycoplasma cynos seems to be associated with respiratory disease.  a0005-9366