02198nas a2200241   4500000000100000000000100001008004100002260007000043653001000113653001600123653000900139653000700148653000900155653001800164100001400182700001700196700001400213245011300227300001200340490000800352520158200360022001401942       2014                            d  c07/2014bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover10ablood10acoagulation10abird10aPT10aaPTT10athrombin time1 aV Guddorf1 aN Kummerfeld1 aR Mischke00aMethodical aspects of blood coagulation measurements in birds applying commercial reagents – a pilot study  a322-3270 v1273 aThe aim of this study was to examine the suitability of commercially available reagents for measurements of coagulation parameters in citrated plasma from birds. Therefore, plasma samples of 17 healthy donor birds of different species were used to determine prothrombin time (PT), activated partial thromboplastin time (aPTT) and thrombin time (TT) applying various commercial reagents which are routinely used in coagulation diagnostics in humans and mammals. A PT reagent based on human placental thromboplastin yielded not only shorter clotting times than a reagent containing recombinant human tissue factor (median 49 vs. 84 s), but also showed a minor range of distribution of values (43–55 s vs. 30–147 s, minimum–maximum, n = 5 turkeys). An aPTT reagent containing kaolin and phospholipids of animal origin delivered the shortest clotting times and the lowest range of variation in comparison to three other reagents of different composition. However, even when this reagent was used, aPTTs were partially extremely long ( gt;200 s). Thrombin time was 38 s (28–57 s, n = 5 chicken) when measured with bovine thrombin at a final concentration of 2 IU thrombin/ ml. Coefficients of variation for within-run precision analysis (20 repetitions) of PT was 8.0% and 4.7% for aPTT measurements using selected reagents of mammalian origin. In conclusion, of the commercially available reagents tested, a PT reagent based on human placental thromboplastin and an aPTT reagent including rabbit brain phospholipid and kaolin, show some promise for potential use in birds.   a0005-9366