01528nas a2200229 4500000000100000000000100001008004100002260007000043653001500113653001700128653001800145100002600163700001600189700001200205700001800217700001800235245009300253300001200346490000800358520091600366022001601282 2014 d c06/2014bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover10aprevalence10abreeding cat10areal-time PCR1 aK Płoneczka-Janeczko1 aK Bierowiec1 aJ Bania1 aM Kiełbowicz1 aZ Kiełbowicz00aFelid herpesvirus 1 (FHV 1) carriers among urban breeding facilities in Wroclaw (Poland) a243-2460 v1273 aThe prevalence of Felid herpesvirus 1 (FHV 1) carriers among closed, one-cat breeding facilities in the urban environment of Wrocław city between 2011-2013 was investigated. A probe-based real-time PCR was used to detect FHV 1 DNA in conjunctival swab extracts. Of the 67 breeding cats investigated 14 animals (20.9%) were confirmed to contain FHV 1 specific DNA. The herpesvirus DNA was detected in eight cats from the group expressing clinical signs of FHV 1 infection (n = 28) and in six clinically healthy animals (n = 39). The percentage of positive carriers ranged from 12 to 44%, depending on the frequency of sampling. The total prevalence in cats at the optimal age for reproduction (2 to 7 years) was 20%. One-cat breeding facilities of purebred cats in Wrocław (Poland) constitute a significant reservoir of FHV 1 and the number of monitoring repeats may influences the success of FHV 1 detection.  a0005-9366