01385nas a2200205   4500000000100000000000100001008004100002260004400043100001200087700002100099700001300120700001000133700001200143700001200155245011200167300001200279490000800291520086600299022001401165       2007                            d  c09/2007bM. & H. Schaper GmbHaHannover1 aA Burgu1 aO Sarimehmetoglu1 aB Gonenc1 aH Oge1 aG Sahin1 aS Aypak00aEffects of Immunization with Purified specific Proteins on the Formation of secondary hydatid Cysts in Mice  a349-3530 v1143 aIn this study, immunoreactive proteins (8, 68 and 116 kDa) of Echinococcus granulosusmetacestode stages were partially purified using isoelectrical focusing by aRotofor cell device. Sixty Swiss albino mice were separated into three experimentaland three control groups (n = 10). The mice of the three experimental groupswere immunized with 8, 68 and 116 kDa purified proteins, respectively. 25 daysafter immunization, serum samples from each group were examined to assessimmunization status by Western blotting. Protoscolices were applied intraperitoneallyto control and immunized groups. By necropsy, mice were examined forcyst formation, size, number and fertility 2, 5, 8, and 10 months after experimentalinfection, respectively. Immunization with all three proteins was found to have asignificant inhibitory effect on the metacestode formation of in mice (p  a0341-6593