01870nas a2200241   4500000000100000000000100001008004100002260007000043653001000113653002600123653001500149100001500164700001100179700002500190700001600215700001300231700001300244245006900257300001200326490000800338520126800346022001401614       2012                            d  c02/2012bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover10aswine10aphylogenetic analysis10agenotype 31 aD Zwettler1 aM Fink1 aS Revilla-Fernández1 aA Steinrigl1 aP Winter1 aJ Köfer00aFirst detection of Hepatitis E virus in Austrian pigs by RT-qPCR  a281-2890 v1253 aLong known to cause disease outbreaks in man in countries with poor sanitary conditions, an increasing number of autochthonous HEV genotype 3 infections have been reported in industrialised countries. Genotype 3 poses an important potential zoonotic threat, with infected pigs functioning as the main reservoir. This study reports the first detected emergence of HEV in Austrian pigs. Five Austrian strains were partially sequenced and phylogenetic analysis demonstrates that they cluster within genotype 3. In addition, a reverse transcription quantitative real-time PCR (RT-qPCR) method using a MGB-hydrolysis probe was developed offering the possibility to detect the HEV genotype 3 in faeces, liquid- and tissue-samples from domestic pigs. The method was adapted to the strains found in Austria. Sensitivity of the assay was tested with different pig organs (liver, mesenteric lymph nodes and kidney) as well as with serum, bile and faeces samples. Within the dynamic range of the assay, a quantitative determination of virus loads was performed. For specificity testing several common swine pathogens were used. Results demonstrated that the proposed method allows implementation of reliable high-throughput screening of Austrian swine samples in the future.  a0005-9366