01694nas a2200229   4500000000100000000000100001008004100002260007000043653001100113653001800124653001800142653001600160653001500176100001400191700001400205700001700219700001500236245017700251490000800428520101400436022001401450       2019                            d  c10/2019bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover10asnails10apentobarbital10apremedication10aanaesthesia10aeuthanasia1 aJ Ziętek1 aA Wójcik1 aS Winiarczyk1 aŁ Adaszek00aThe use of sodium pentobarbital intravascular injections in the premedication, anaesthesia and euthanasia of Cornu aspersum maximum for research purposes – in-house study0 v1323 aThe aim of this article is to present the authors’ own method of using pentobarbital for premedication, anaesthesia or euthanasia of a snail, depending on the dose.  Premedication and anaesthesia facilitate the procedures in studies using snails as laboratory animals. Different procedures, for example haemolymph collection and probing is much easier in anaesthetized animals. The research of the authors resulted in developing a complete procedure of drug administration directly to the circulatory system of Cornu aspersum maximum with high precision of dosing. C. aspersum maximum is a farm animal used as a source of meat in the food industry and can also be an alternative model for experiments requiring the use of animals. It is worth emphasizing that snails as invertebrates are not subject to the regulations of animal research ethics committees in the EU. However, it should be emphasized that it is a humanitarian method and as a way of premedication and anaesthesia-completely safe for animals.  a0005-9366