02118nas a2200241   4500000000100000000000100001008004100002260007000043653002500113653003100138653002700169653000800196100001200204700001300216700001800229700001400247700001600261700001500277245013400292490000800426520142800434022001401862       2019                            d  c02/2019bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover10aBlack soldier larvae10amicrobial characterization10amicrobial inactivation10aHHP1 aC Marin1 aP Urbina1 aP Salvatierra1 aD Rodrigo1 aP Fernandez1 aA Martinez00aEffect of High Hydrostatic Pressure on natural contaminating microorganisms and inoculated Salmonella in Hermetia illucens larvae0 v1323 aA microbiological characterization of black soldier fly (Hermetia illucens) larvae as well  as an inactivation study of naturally contaminating microorganisms and inoculated Salmonella enteriditis var. Typhimurium in these larvae by using High Hydrostatic  Pressure (HHP; 300 to 350 MPa, for 1.5 to 15 min) was carried out. Larvae had a total aerobic mesophilic bacteria (AMB) load of 5.28 log cfu/g and an Enterobacteriaceae  load of 4.29 log cfu/g. The presence of pathogenic microorganism varied: no Listeria spp. were found, but Salmonella spp and Escherichia (E.) coli were detected  in the larvae extract at a contamination level of 4.04 log cfu/g and 3.84 log cfu/g, respectively. The total anaerobic bacteria load was 5.63 log cfu/g, but no sulfitereducing  Clostridium were encountered. The mold and yeast level was 5.19 log cfu/g. HHP was effective against naturally contaminating yeasts and molds producing  reductions by around 3 log cycle at 350 MPa for 7 min of treatment, but a low reduction of AMB was achieved, even at the highest pressure level (350 MPa for 15  min). The inactivation level of Salmonella inoculated in larvae varied. At 350 MPa for 7 min, more than 3 log cycle reductions were achieved. Inactivation curves were fitted  to different mathematical models. Among the three inactivation models studied, the one that best described the inactivation pattern of the cells was the biphasic model.  a0005-9366