01972nas a2200265   4500000000100000000000100001008004100002260007000043653002200113653001200135653002700147653002800174100001600202700001400218700001800232700001400250700001700264700001600281700001600297245009900313300001200412490000800424520126000432022001401692       2015                            d  c03/2015bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover10aChlamydia abortus10aCroatia10aBosnia and Herzegovina10aovine enzootic abortion1 aS Špičić1 aI Račić1 aM Andrijanić1 aS Duvnjak1 aM Zdelar-Tuk1 aM Stepanić1 aŽ Cvetnić00aEmerging cases of chlamydial abortion in sheep and goats in Croatia and Bosnia and Herzegovina  a183-1870 v1283 aIn a recent lambing season (2012/2013), the seroprevalence of ovine chlamydiosis was monitored in small ruminant abortion cases in Croatia. Blood samples of 93 sheep and 69 goats were examined. In addition, 50 sheep and 61 goat samples were tested using molecular methods. Furthermore, 14 sheep blood samples, one goat blood sample and one sheep placenta sample from Bosnia and Herzegovina (BIH) were also tested as a part of inter-laboratory cooperation. Overall high seroprevalence was detected in sheep, 19.6% with the ELISA IDEXX kit and 20.5% with the CIVTEST kit. Seroprevalence in goats was 11.4%. In BIH, four sheep and one goat blood sample were seropositive for chlamydiosis. The disease causing agent, Chlamydia abortus (C. abortus) was confirmed using molecular methods in two sheep flocks in continental Croatia and in one sheep flock in BIH. In this study, C. abortus infection in sheep was identified for the first time in Croatia using species specific molecular methods. Ovine chlamydiosis is present in national sheep and goat flocks in Croatia and BIH. Thus should be subject to ongoing controls in the case of abortion. A combination of serological and molecular methods should be used for optimal laboratory diagnostics of C. abortus.  a0005-9366