01647nas a2200217   4500000000100000000000100001008004100002260007000043100001500113700001500128700001500143700001500158700001300173700001300186700001400199245005800213300001200271490000800283520112400291022001401415       2012                            d  c05/2012bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover1 aC Freuling1 aB Hoffmann1 aT Selhorst1 aF Conraths1 aJ Kliemt1 aJ Schatz1 aT Müller00aNew insights into the genetics of EBLV-1 from Germany  a259-2630 v1253 aPrevious epidemiological studies on EBLVs indicated a distinct geographical distribution of EBLV-1 in Germany. In this study, 48 isolates were selected to further investigate the spatial and temporal distribution of EBLV-1 variants in Germany. The nucleoprotein-gene (N), the nucleoprotein-phosphoprotein spanning untranslated region (NP-UTR) and the UTR between G- and L-gene of each isolate were sequenced using direct cycle sequencing. Results of the subsequent phylogenetic analysis of the N-gene confirmed previous studies on EBLVs, showing a high sequence identity among German EBLV-1a isolates, and a correlation between genetic and temporal and spatial distance, respectively, was shown. Our results indicate that the GL-UTR is not suitable for phylogenetic analyses. Interestingly, 6 nt insertions in two isolates as well as a single nucleotide insertion in a different isolate were detected in the N-P UTR. Within the UTR between Gand L-gene one isolate showed a 35 nt deletion. The effect of those changes on viral properties remains elusive as such mutations have not been described for lyssaviruses before.  a0005-9366