01988nas a2200253   4500000000100000000000100001008004100002260007000043653002700113653001700140653002200157653001900179653002300198100001500221700001400236700002200250700001600272700001200288700001800300245016000318490000800478520123400486022001401720       2019                            d  c02/2019bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover10aSalmonella Senftenberg10aEnterococcus10aAscaris suum eggs10asurvival times10apig slurry hygiene1 aZ Paluszak1 aK Skowron1 aJ Bauza-Kaszewska1 aH Olszewska1 aG Gryń1 aM Lukaszewicz00aInactivation of indicator microorganisms in pig slurry mixed with maize silage during anaerobic digestion at different temperatures – preliminary results0 v1323 aThe aim of the study was to evaluate the sanitary (hygienic) effect of meso- and thermophilic anaerobic digestion on Salmonella Senftenberg W775 H2S-negative, enterococci and Ascaris suum eggs used as indicator organisms in pig slurry mixed with maize. To assess the effect of the digestion process on indicator bacteria number the method of indirect monitoring was applied. For this purpose special carriers providing isolation of the microorganisms from the external contamination, but not from physical and chemical conditions in the bioreactor were used. The rate of inactivation of the indicator organisms was significantly higher during thermophilic than mesophilic fermentation of the slurry. The survival times of Salmonella Senftenberg and enterococci, calculated from the experimental data, ranged from 25 to 31 hours. Invasive A. suum egg forms were completely inactivated after 2 hours. Mesophilic fermentation resulted in considerably longer survival times between 10 and 15 days for both bacteria and parasite eggs as calculated from the experimental data. The presented method may be used for the development of a new strategy for evaluating the sanitary and hygienic status of anaerobically fermented pig slurry.   a0005-9366