02092nas a2200301   4500000000100000000000100001008004100002260007000043653003900113653003200152653002400184653001700208653002100225653002600246653001000272100001200282700001300294700001700307700001500324700001200339700001400351700001200365245021200377300001000589490000800599520116900607022001401776       2012                            d  c01/2012bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover10aCorynebacterium pseudotuberculosis10arecombinant phospholipase D10awhole cell antigens10aimmunisation10aprevalence study10acaseous lymphadenitis10aELISA1 aR Sting1 aB Wagner1 aA Sari-Turan1 aM Stermann1 aM Reule1 aM Eichner1 aW Beyer00aSerological studies on Corynebacterium pseudotuberculosis infections in goats in Baden-Wuerttemberg (Germany) and seroreactions on antigens used for newly developed Enzyme-Linked Immunosorbent Assays (ELISA)  a67-750 v1253 aIn the present study, comprehensive data on the seroprevalence of Corynebacterium (C.) pseudotuberculosis infections in goats are presented for Baden-Wuerttemberg, a Federal State of Germany, for the first time. As a prerequisite, ELISAs based on a recombinant phospholipase D (rPLD) and whole cell antigens (WCA) were designed and validated yielding sensitivity values of 81% and 97% and specificity values of 98% and 99%, respectively. Immunisation trials in goats demonstrated a significant production of antibodies to rPLD but an evidently lower antibody production to WCA as determined in the corresponding ELISA. Moreover, immunisation with rPLD resulted in the formation of antibodies, which were also detected in the WCA ELISA. In contrast, this phenomenon was not observed with the rPLD ELISA after immunisation with WCA. Implementation of the rPLD and WCA ELISAs in a broad-based seroprevalence study in Baden-Wuerttemberg revealed positive reactions in both ELISAs in 13.2% of the 1771 goat sera tested. In 53.7% of 121 herds of which five or more animals were tested per herd there was at least one animal that showed a positive reaction in both tests.  a0005-9366