02206nas a2200313   4500000000100000000000100001008004100002260007000043653003300113653001100146653001700157653001300174653000800187653002000195100001300215700001800228700001200246700001200258700001500270700001900285700001200304700001600316700002600332245008000358300001000438490000800448520142200456022001401878       2014                            d  c02/2014bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover10aArcanobacterium haemolyticum10adonkey10aMALDI-TOF MS10a16S rDNA10agap10avirulence genes1 aO Sammra1 aA Balbutskaya1 aS Nagib1 aJ Alber1 aC Lämmler1 aA Abdulmawjood1 aM Timke1 aM Kostrzewa1 aE Prenger-Berninghoff00aProperties of an Arcanobacterium haemolyticum strain isolated from a donkey  a56-600 v1273 aThe present study was designed to characterize phenotypically and genotypically an Arcanobacterium haemolyticum strain (A. haemolyticum P646) isolated from a purulent nasal discharge of a donkey. A. haemolyticum P646 showed, compared to sheep blood, an enhanced hemolytic reaction on rabbit blood agar, a synergistic CAMP-like reaction with Streptococcus agalactiae and Rhodococcus equi as indicator strains, a reverse CAMP reaction in the zone of Staphylococcus aureus β-hemolysin and the typical biochemical properties of this species. The species identity could be confirmed by MALDI-TOF MS analysis, by sequencing the 16S rDNA and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap and by amplification of A. haemolyticum specific parts of 16S-23S rDNA intergenic spacer region and 23S rDNA. A. haemolyticum P646 and the reference strain A. haemolyticum DSM 20595 were further characterized by amplification of the putative virulence genes encoding arcanolysin, phospholipase D, hemolysin A, CAMP factor family protein, a collagen binding protein and two neuraminidases which were present for A. haemolyticum DSM 20595. A. haemolyticum P646 showed a comparable gene spectrum but was negative for the genes encoding collagen binding protein and neuraminidase H. To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. haemolyticum strain isolated from a donkey.  a0005-9366