02451nas a2200277   4500000000100000000000100001008004100002260007000043653002000113653001500133653001000148653001300158100001200171700001400183700001700197700001500214700001300229700001200242700001200254700001400266700001700280245013500297490000800432520171900440022001402159       2018                            d  c03/2018bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover10aFluoroquinolone10aresistance10aroute10atransfer1 aE Burow1 aM Grobbel1 aB-A Tenhagen1 aC Simoneit1 aM Ladwig1 aI Szabo1 aD Wendt1 aS Banneke1 aA Käsbohrer00aAntimicrobial susceptibility in faecal Escherichia coli from pigs after enrofloxacin administration in an experimental environment0 v1313 aThe study objective was to evaluate the effect of oral (OT) and parenteral (PT)  administration of enrofloxacin to weaners on untreated contact animals. We  assessed a) fluoroquinolone occurrence in the blood serum of untreated contact  animals (COT, CPT); b) resistance to (fluoro)quinolones in commensal Escherichia  coli (E. coli) in OT, PT, COT and CPT compared to the control (CON), and c)  resistance to other antimicrobials in E. coli in OT, PT, COT and CPT compared  to the initial situation before the treatment in these groups. Five groups of 14  weaners each were housed in three separate rooms (OT with COT, PT with CPT,  CON alone). OT and PT were treated with enrofloxacin for five days. Rectal swabs  and blood samples were taken before, during and until 51 days after treatment.  Enro- and ciprofloxacin were detected in all treated, all COT and half of the CPT  pigs. Neither through selective isolation nor by susceptibility testing of one  random non-selectively isolated faecal E. coli per sample, resistance to ciprofloxacin  (metabolite of enrofloxacin) and nalidixic acid was detected in both treatment  and contact groups during and short after treatment. However, a transient  increase of E. coli resistant to antimicrobials other than quinolones followed the  treatment in isolates from OT and COT (e.g. ampicillin p  lt; 0.05). In conclusion,  animals in contact with treated animals are exposed to and can intake antimicrobials.  Animals in contact with orally treated animals show occurrence of antibiotic  resistant E. coli. Further studies are needed to show whether these preliminary  findings can be confirmed under different conditions and with more sensitive  detection methods.   a0005-9366