01413nas a2200241   4500000000100000000000100001008004100002260007000043653001300113653000900126100001500135700001500150700001300165700001600178700002000194700001200214700001800226245006900244300001200313490000800325520082400333022001401157       2013                            d  c11/2013bSchlütersche Verlagsgesellschaft mbH & Co. KGaHannover10a23S rRNA10akmt11 aS Adhikary1 aM Bisgaard1 aG Foster1 aN Kiessling1 aA Rise Fahlén1 aJ Olsen1 aH Christensen00aComparative study of PCR methods to detect Pasteurella multocida  a415-4220 v1263 aThe aim of the investigation was to compare four published PCR methods for specific detection of Pasteurella multocida. 85 strains of P. multocida and 13 strains of other taxa were included in the comparison of four published PCR methods based on kmt1, 23S rRNA and transcriptional regulator genes pm0762 and pm1231 as targets, respectively. Sensitivities were calculated to 100 and 80% for the kmt1 and 23S rRNA based PCRs, respectively and specificities to 92 and 23%, respectively of the kmt1 and 23S rRNA based PCRs. The methods based on transcriptional regulator genes tested two Bisgaard taxon 45 strains positive in addition to one Bisgaard taxon 16 strain. Only the kmt1 method detected P. multocida with the expected sensitivity and specificity and this method is recommended for identification of the species.  a0005-9366