@article{4157,
  keywords = {PCR, RFLP, primer, animals, diagnostics},
  author = {Z Sihelská and T Csank and E Čonková and M Harčárová and P Váczi},
  title = {PCR-RFLP for identification of animal Malassezia species in mammals},
  abstract = {The genus Malassezia comprises at least 16 species, from which 11 species are occurring in animals (9 species in mammals and 2 species in birds). The diagnostics of Malassezia is based on phenotypic and genotypic identification. The phenotypic examination is not sufficiently sensitive and exact and therefore the molecular methods are necessary for accurate identification. A range of molecular methods have been developed for the diagnostics of Malassezia species, but there are several disadvantages associated with them, such as inefficiency of differentiating all species, technically difficult, high cost and questionable reproducibility. No simple method is available that allows identification of all animal Malassezia species in mammals. A new PCR-RFLP method with primers MalasF (5´-GAGCATGCCTGTTTGAGTGCC-3´) and MalasR (5´-GCAAATGACGTATCATGCCAT-3´) and with the enzymes MspI, EcoRI and PflMI was especially developed for the detection of nine animal Malassezia species in mammals (M. furfur, M. globosa, M. slooffiae, M. sympodialis, M. pachydermatis, M. caprae, M. equina, M. cuniculi and M. nana). The method is technically simple and capable of identifying Malassezia species of animals with high accuracy and reliability.},
  year = {2017},
  journal = {Berliner und Münchener Tierärztliche Wochenschrift},
  volume = {130},
  month = {11/2017},
  publisher = {Schlütersche Verlagsgesellschaft mbH & Co. KG},
  address = {Hannover},
  issn = {0005-9366},
  doi = {10.2376/0005-9366-17035},
  language = {English},
}