@article{4101,
  keywords = {Anaplasma phagocytophilum, tick-borne diseases, PCR, wild bison},
  author = {B Dzięgiel and Ł Adaszek and M Krzysiak and M Skrzypczak and M Adaszek and B Furmaga and S Winiarczyk},
  title = {The occurrence of Anaplasma phagocytophilum in wild bison from the Bialowieza Primeval Forest in Eastern Poland},
  abstract = {The aim of the present study was to investigate the occurrence of Anaplasma spp. in a group of 120 wild bison (Bison bonasus) from the Bialowieza Primeval Forest in eastern Poland and to determine which species of Anaplasma could infect these animals based on a PCR of a part of the 16S rRNA gene followed by sequencing. The PCR technique showed the presence of 16S rRNA Anaplasma spp. genetic material in the blood of 22 from a total of 120 animals. DNA amplification by means of the primers EHR 521 and EHR 747 gave a product size of 252-bp. The sequences of the PCR products obtained showed 100% homology with each other and 100% homology with the Anaplasma phagocytophilum GU 18908 sequence from our earlier study, isolated from a horse with a clinical case of anaplasmosis. The similarity of the sequences with the sequences of the 16S rRNA gene isolated from various Anaplasma species deposited in the GeneBank, ranged between 95.8% and 98.8%. Based on the results of molecular analysis, bacterial DNA detected in the blood of 22 wild bison was identified as Anaplasma phagocytophilum. },
  year = {2015},
  journal = {Berliner und Münchener Tierärztliche Wochenschrift},
  volume = {128},
  edition = {7/8},
  pages = {310-314},
  month = {07/2015},
  publisher = {Schlütersche Verlagsgesellschaft mbH & Co. KG},
  address = {Hannover},
  issn = {0005-9366},
  doi = {10.2376/0005-9366-128-310},
  language = {English},
}