@article{3856,
  author = {B Makoschey and M Beer},
  title = {A live Bovine Herpesvirus-1 marker vaccine is not shed after intramuscular vaccination},
  abstract = {It should be established, whether animals vaccinated intramuscularly (IM) with a live Bovine herpesvirus type 1 (BHV-1) marker vaccine become viremic and/or excrete vaccine virus with nasal discharge. Five cattle, seronegative for BHV-1, were vaccinated with an overdose of the vaccine (BovilisR IBR marker live) via the IM route. Nasal swabs and blood samples were taken at regular intervals and tested for BHV -1 in a virus infectivity assay. In addition, a polymerase chain reac-tion (PCR) specific for BHV-1 DNA was performed on the blood samples. BHV-1 neutralizing antibody titres were determined in the sera taken prior to the vacci-nation and four weeks after immunisation. All animals were successfully vaccina-ted as judged by the development of BHV -1 neutralising antibodies. However, all nasal swab samples were tested negative for vaccine virus, and all blood samples were found negative for BHV-1 vaccine virus and BHV-1 specific DNA. From these data it can be concluded that the vaccine virus was not excreted with nasal discharge after IM vaccination and that the vaccinated animals did not have a detectable viremia. Therefore, it is recommended to apply the tested BHV-1 marker live vaccine by the IM route in situations where it is undesirable that the vacci-ne virus is excreted.},
  year = {2007},
  journal = {Berliner und Münchener Tierärztliche Wochenschrift},
  volume = {120},
  pages = {480-482},
  month = {11/2007},
  publisher = {Schlütersche Verlagsgesellschaft mbH & Co. KG},
  address = {Hannover},
  issn = {0005-9366},
  doi = {10.2376/0005-9366-120-480},
  language = {English},
}