@article{3467,
  keywords = {antibodies, neutrophils, selectin, testis, torsion},
  author = {M Celebi and  and A Paul},
  title = {Blockade of p-selectin reduces neutrophil infiltration into the murine testis after ischemia-reperfusion-injury},
  abstract = {Germ cell specific apoptosis after ischemia-reperfusion (I/R) induced testicular injury is dependent on neutrophil recruitment to the testis. Intravascular adhesion molecules like the P- and E- selectins play an important role in this recruitment. The purpose of this study was to inhibit neutrophil recruitment in I/R induced testicular injury by using a function-blocking monoclonal anti-mouse P-selectin antibody. Adult mice were subjected to a 2 h period of testicular torsion (ischemia) followed by detorsion (reperfusion). Ten minutes after the onset of reperfusion, mice received either 100  amp;#956;g of a function-blocking monoclonal P-selectin antibody (FBMAB group) or isotype-matched control antibody (IMCA group). Separate groups of mice underwent sham-operation (SO group) or received 500 ng of TNFM (IF group) to induce inflammation. Mice were sacrificed 24 h after reperfusion and testiscular interstitial cells were isolated and analyzed for the presence of neutrophils by means of flow cytometry. The function-blocking monoclonal P-selectin antibody reduced neutrophil recruitment in I/R induced testicular injury significantly (FBMAB group as compared to the IMCA group 26 ± 4 vs. 52 ± 10% Gr #150;1+CD11b+ of total leucocytes; P  lt; 0.001). Therefore, blocking P-selectin may be therapeutically beneficial to protect postischemic testis. },
  year = {2008},
  journal = {Berliner und Münchener Tierärztliche Wochenschrift},
  volume = {115},
  pages = {457-460},
  month = {12/2008},
  publisher = {M. & H. Schaper GmbH},
  address = {Hannover},
  issn = {0341-6593},
  doi = {10.2376/0341-6593-115-457},
  language = {English},
}