@article{3311,
  author = {R Sting and M Stermann},
  title = {Duplex real-time PCr assays for rapid detection of virulence genes in E. coli isolated from post-weaning pigs and calves with diarrhoea},
  abstract = {Duplex realtimePCR assays were used as modules to cover partially automateddetection of 12 genes encoding adhesins, enterotoxins and Shiga toxins in faecalE. coli isolates. For this a total of 194 E. coli isolates from pigs suffering frompostweaningdiarrhoea (PWD), including 65 isolates with haemolytic activity,and 83 isolates from calves with diarrhoea were examined. Data obtained by PCRwere compared with Otypingand with haemolytic activity as indirect virulencemarkers. E. coli OtypesO139:K82, O141:K85, and O149:K91 accounted for 43.8 %(n = 85) of all porcine strains and for 55.4 % (n = 36) of the porcine strains, whichexhibited haemolytic activity. These strains carried virulence genes by 65.9 %(n = 56) and 80.6 % (haemolytic E. coli, n = 29), respectively. The E. coli OtypesO139:K82 and O141:K85 were significantly associated with the adhesin gene F18,and O149:K81 with the F4 gene. In this context, detection of the gene encodingF18 was coupled predominantly with the genes responsible for the production ofthe toxins STI,STIIand Stx2, and the F4 gene with those of the enterotoxins STI,STIIand LT. Both virulence patterns were detected more pronounced in E. colistrains with haemolytic activity.Fiftysixof a total of 83 E. coli isolates originating from calves were OtypedasO101 (O101:K28, O101:K30, O101:K32; n = 29), O78:K80 (n = 23), and O9:K35 (n = 4).Most of the E. coli O78:K80 strains carried the F17 gene (69.6 %, n = 16). Virulencegenes encoding for F4, F5 or STIwere detected only in single cases. Intimin andShiga toxin genes that are present in enterohaemorrhagic E. coli (EHEC) were notdetected.},
  year = {2008},
  journal = {Berliner und Münchener Tierärztliche Wochenschrift},
  volume = {115},
  pages = {231-238},
  month = {06/2008},
  publisher = {M. & H. Schaper GmbH},
  address = {Hannover},
  issn = {0341-6593},
  doi = {10.2376/0341-6593-115-231},
  language = {English},
}