@article{2560,
  keywords = {Hepatitis contagiosa canis, HCC, infectious canine hepatitis, ICH, epizootic fox encephalitis, wild carnivores},
  author = {C Schulze and J Schatz and E Dohrmann and P Wohlsein},
  title = {Molecular epidemiology of canine adenovirus type 1 (CAdV-1) in free-ranging small carnivores in the Berlin-Brandenburg region, Germany. A preliminary study},
  abstract = {To date, there is no actual knowledge about the molecular epidemiology of  canine adenovirus type 1 (CAdV-1) in free-ranging small carnivores in the federal  states of Berlin and Brandenburg, Germany. However, serological data collected  in this region (1991–1995) indicate that CAdV was at least endemic in red foxes  (Vulpes vulpes) with a seroprevalence of 3.5 %. In our preliminary study, we  examined relevant tissues (liver, kidney, brain) from 98 red foxes and 16 raccoons  (Procyon lotor) submitted in the context of the national rabies monitoring  program by real-time PCR, histopathology and immunohistochemistry. CAdV-1-specific genome sequences were detected in the tissues of 13 out of the 98 red  foxes (13.3 %) with ct-values ranging between 16.0 and 35.8. Immunohistochemically,  CAdV-specific antigen was found in the brain of three red foxes, mainly in  endothelial cells. These red foxes had a non-suppurative meningoencephalitis  indicative of epizootic fox encephalitis by the presence of typical adenoviral intranuclear  inclusion bodies. All raccoons were negative for CAdV-1 and all red foxes  and raccoons were negative for CAdV-2 in the real-time PCR. Our study confirmed  the enzootic occurrence of CAdV-1 in red foxes in the Berlin-Brandenburg region  of Germany emphasizing the need for continuation of the vaccination of susceptible  carnivores kept as pets or in zoological gardens. The actual detection rate by  PCR was considerably higher than by serology in a previous study. However, the  relevance of this finding has to be evaluated by future serological and molecular  investigations.},
  year = {2019},
  journal = {Berliner und Münchener Tierärztliche Wochenschrift},
  volume = {132},
  month = {03/2019},
  publisher = {Schlütersche Verlagsgesellschaft mbH & Co. KG},
  address = {Hannover},
  issn = {0005-9366},
  doi = {10.2376/0005-9366-18071},
  language = {English},
}