@article{2286,
  author = {C Freuling and B Hoffmann and T Selhorst and F Conraths and J Kliemt and J Schatz and T Müller},
  title = {New insights into the genetics of EBLV-1 from Germany},
  abstract = {Previous epidemiological studies on EBLVs indicated a distinct geographical distribution of EBLV-1 in Germany. In this study, 48 isolates were selected to further investigate the spatial and temporal distribution of EBLV-1 variants in Germany. The nucleoprotein-gene (N), the nucleoprotein-phosphoprotein spanning untranslated region (NP-UTR) and the UTR between G- and L-gene of each isolate were sequenced using direct cycle sequencing. Results of the subsequent phylogenetic analysis of the N-gene confirmed previous studies on EBLVs, showing a high sequence identity among German EBLV-1a isolates, and a correlation between genetic and temporal and spatial distance, respectively, was shown. Our results indicate that the GL-UTR is not suitable for phylogenetic analyses. Interestingly, 6 nt insertions in two isolates as well as a single nucleotide insertion in a different isolate were detected in the N-P UTR. Within the UTR between Gand L-gene one isolate showed a 35 nt deletion. The effect of those changes on viral properties remains elusive as such mutations have not been described for lyssaviruses before.},
  year = {2012},
  journal = {Berliner und Münchener Tierärztliche Wochenschrift},
  volume = {125},
  pages = {259-263},
  month = {05/2012},
  publisher = {Schlütersche Verlagsgesellschaft mbH & Co. KG},
  address = {Hannover},
  issn = {0005-9366},
  doi = {10.2376/0005-9366-125-259},
  language = {English},
}