@article{2259, keywords = {Salmonella Senftenberg, Enterococcus, Ascaris suum eggs, survival times, pig slurry hygiene}, author = {Z Paluszak and K Skowron and J Bauza-Kaszewska and H Olszewska and G Gryń and M Lukaszewicz}, title = {Inactivation of indicator microorganisms in pig slurry mixed with maize silage during anaerobic digestion at different temperatures – preliminary results}, abstract = {The aim of the study was to evaluate the sanitary (hygienic) effect of meso- and thermophilic anaerobic digestion on Salmonella Senftenberg W775 H2S-negative, enterococci and Ascaris suum eggs used as indicator organisms in pig slurry mixed with maize. To assess the effect of the digestion process on indicator bacteria number the method of indirect monitoring was applied. For this purpose special carriers providing isolation of the microorganisms from the external contamination, but not from physical and chemical conditions in the bioreactor were used. The rate of inactivation of the indicator organisms was significantly higher during thermophilic than mesophilic fermentation of the slurry. The survival times of Salmonella Senftenberg and enterococci, calculated from the experimental data, ranged from 25 to 31 hours. Invasive A. suum egg forms were completely inactivated after 2 hours. Mesophilic fermentation resulted in considerably longer survival times between 10 and 15 days for both bacteria and parasite eggs as calculated from the experimental data. The presented method may be used for the development of a new strategy for evaluating the sanitary and hygienic status of anaerobically fermented pig slurry. }, year = {2019}, journal = {Berliner und Münchener Tierärztliche Wochenschrift}, volume = {132}, month = {02/2019}, publisher = {Schlütersche Verlagsgesellschaft mbH & Co. KG}, address = {Hannover}, issn = {0005-9366}, doi = {10.2376/0005-9366-18024}, language = {English}, }