@article{1523,
  keywords = {Corynebacterium pseudotuberculosis, ELISA, recombinant phospholipase D, whole-cell antigens},
  author = {R Sting and L Schneider-Bühl and H Wagner and J Maget and B Polley and D Bürstel and H Axt},
  title = {Clinical and serological investigations on caseous lymphadenitis in goat breeding herds  in Baden-Wuerttemberg},
  abstract = {Data on clinical and serological examinations were collected within the first step of a programme for control of caseous lymphadenitis in goat breeding herds in Baden-Wuerttemberg, a federal state of Germany. For the serological studies, an in-lab ELISA based on whole-cell antigens and a commercially available ELISA based on recombinant phospholipase D were evaluated, revealing sensitivity values of 85%−91% and specificity values of 93%−100%, respectively, depending on the cut-off value defined. Comparative evaluations on the agreement between the results of both ELISAs reached kappa values of nearly 0.9 and a proportion of agreement of about 95%. A total of 1333 goats from 27 herds were examined clinically and blood samples were taken from each animal for serological testing. Using the in-lab and the commercially available ELISA test, 5.3% (n = 71) and 4.8% (n = 64) of animals originating from nine and five herds revealed positive results, respectively. In clinical examinations, 5.2% (n = 69) of goats from 12 different herds displayed palpable swelling of lymph nodes, particularly affecting the Ln. cervicalis superficialis (n = 30), followed by the Ln. mandibularis (n = 11), Ln. inguinalis superficialis (n = 9), Ln. popliteus (n = 9), Ln. parotideus (n = 5), and the Ln. retropharyngeus (n = 2). Results of clinical positive and serological positive animals differed. However, clinically apparent goats revealed a significant higher rate of serologically positive results than clinically unapparent animals.},
  year = {2017},
  journal = {Berliner und Münchener Tierärztliche Wochenschrift},
  volume = {130},
  edition = {3/4},
  pages = {133-143},
  month = {03/2017},
  publisher = {Schlütersche Verlagsgesellschaft mbH & Co. KG},
  address = {Hannover},
  issn = {0005-9333},
  doi = {10.2376/0005-9366-16024},
  language = {English},
}