@article{1502,
  keywords = {Campylobacter jejuni, microbial community, PCR-SSCP, sequencing, chicken},
  author = {D Sofka and A Pfeifer and B Gleiß and P Paulsen and F Hilbert},
  title = {Changes within the intestinal flora of broilers by colonisation with Campylobacter jejuni},
  abstract = { In most European countries human campylobacteriosis is the most important  bacterial zoonotic foodborne infection. Chicken meat is considered the main  source of infection. Since most strategies assessed so far, in reducing Campylobacter  colonization in chickens or in the reduction of human disease, have not been  very effective, new knowledge regarding Campylobacter’s interaction with the  host is needed. In this study we analysed fecal and cecal samples of five chicken  flocks of different Austrian farms for the occurrence of C. jejuni and C. coli, and  analysed the intestinal microbiota by PCR-SSCP, cultural detection of lactic acid  bacteria, Enterococci, Staphylococci, Enterobacteriaceae, E. coli, and total aerobic colony counts. Furthermore ten chicken samples of cecal content of a flock  during colonization with Campylobacter spp. was analysed by high throughput  sequencing. With all three methods used we could detect a change within the  microbiota caused by C. jejuni. Enumeration of different bacteria was significantly  lower in fecal samples positive for C. jejuni, pointing out that a higher water content  and thus, a preliminary stage of diarrhea might appear during Campylobacter  colonization. By PCR-SSCP analysis the microbiota composition differed between  colonized and non-colonized chicken fecal samples. This could also be detected  in community analysis by high throughput sequencing, but this difference was  only a tendency and not statistically significant. It can be concluded that C. jejuni  is interacting with the intestinal microflora in their respective hosts and hence,  this has to be taken into account when providing new strategies to combat  Campylobacter colonization and disease. },
  year = {2015},
  journal = {Berliner und Münchener Tierärztliche Wochenschrift},
  volume = {128},
  pages = {104-110},
  month = {01/2015},
  publisher = {Schlütersche Verlagsgesellschaft mbH & Co. KG},
  address = {Hannover},
  issn = {0005-9366},
  doi = {10.2376/0005-9366-128-104},
  language = {English},
}